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Identifier

000369226

Title

Drosophila as a model organism for studying insecticide resistance mechanisms in agricultural pests

Alternative Title

Η Δροσόφιλα ως οργανισμός μοντέλο για τη μελέτη των μηχανισμών ανθεκτικότητας σε εντομοκτόνα των εντόμων οικονομικού ενδιαφέροντος

Author

Παυλίδη, Νένα

Thesis advisor

Βόντας, Ιωάννης

Abstract

Insecticide resistance poses a threat to agricultural output thus, understanding in details the underlying mechanisms by which insects gain resistance to different insecticides remains a challenge for the improvement of insect control in the future. Pyriproxyfen is a juvenile hormone analogue insecticide, effective against a variety of arthropod pests. However, the mode of action in the molecular level remains unknown. The aim of this study is to discover the mechanism of action of pyriproxyfen and/or map genes implicated in pyriproxyfen resistance, performing genome-wide insertional mutagenesis using a Minos transposon-based system in D. melanogaster. The selection of the mutated flies was done applying lethal doses of pyriproxyfen. Initially, the lethal dose was determined at 0,5 ppm (2 times the LC99). However, during the screening, an unexpected high number of flies survived in the estimated lethal concentration and therefore, new set of bioassays were designed, in which the genetic background and the number of the eggs per vial were taken into account. We have not managed to obtain conclusive results so far, therefore, insertional mutagenesis in order to cover 3-5% of D. melanogaster genome remains to be done.

Language

English

Subject

Insertional mutagenesis

Minos element

Minos μεταθετό στοιχείο

Pyriproxyfen

Παρεμβατική μεταλλαξιγένεση

Issue date

2011-11-18

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses