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Identifier

000414791

Title

Μελέτη της βιοαποικοδόμησης φαινόλης από φωτοσυνθετικούς μικροοργανισμούς

Alternative Title

Study of phenol biodegradation by photosynthetic microorganisms

Author

Γιακουμάκης, Γρηγόριος Α.

Thesis advisor

Γανωτάκης, Δημήτριος Φ.

Reviewer

Τσιώτης, Γεώργιος
Χανιωτάκης, Νικόλαος

Abstract

The main objective of this study is the investigation of the ability of three different photosynthetic microorganisms, a genus of filamentous cyanobacteria, Anabaena sp., a unicellular microalgae Thalassiosira weissflogii and a cyanobacterium Spirulina platensis, to remove phenol, an aromatic organic compound. During this study, cells were initially cultivated in various growth media and the best conditions for maximal growth were investigated. In addition, cell growth was measured via the PCV method and fluorescence measures were carried out. For the main objective of this study, phenol removal, various experiments were carried out in the presence or in the absence of a carbon source. In those experiments, phenol removal reached 24,1% for Spirulina platensis (SSM with C), while Thalassiosira weissflogii had no effect phenol removal. Although there was phenol removal by Anabaena sp., the standard deviations of the results were such that further testing is necessary. Furthermore, the study of the photosynthetic mechanism of the cyanobacterium Spirulina platensis has shown that it is stressed by the presence of phenol, when there is carbon in the SSM medium, and moreover the stress is more pronounced when the initial phenol concentration is higher. At the same time, the growth of cell volume did not appear to be affected by the presence of phenol. Stress indications are further increased when the carbon source is missing from the nutrient medium.

Language

Greek

Subject

Accumulation of phenol

Anabaena sp.

Bioremediation.

Spirulina platensis

Thalassiosira weissflogii

Βιοαποικοδόμηση

Φαινόλη

Issue date

2018-03-23