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Identifier

000421107

Title

Απομόνωση και χαρακτηρισμός φυσικών προϊόντων από φωτοσυνθετικούς μικροοργανισμούς

Alternative Title

Isolation and characterization of natural products from photosynthetic microorganisms

Author

Κοκαράκης, Εμμανουήλ Ι.

Thesis advisor

Γανωτάκης, Δημήτριος

Reviewer

Παυλίδης, Ιωάννης
Τσιώτης, Γεώργιος

Abstract

In the present study, the isolation of natural substances producing photosynthetic microorganisms has been studied. In particular, the strains used were a microorganism isolated from the Yofyros River, a cyanobacterium which belongs to the genus Anabaena and the microalgae Βotryococcus braunii. It was understood from the results of the laboratory studies that the microalgae isolated from the Yofyros River is a eukaryotic microorganism. We have concluded from its way of division, morphological and biochemical characteristics that this microalgae belongs to the genus Chlorella. This conclusion is also reinforced by the fact that the matrix it produces, contains glucosamine. Glucosamine is a carbohydrate commonly found in the cell wall of the genus Chlorella. In addition, through the transmission electron microscopy (TEM) and scanning electron microscopy (SEM) techniques it was found that the matrix produced by the microorganism uses it as a protective mantle to separate it. The matrix of the microorganism appears macroscopically as a white solid. Isolation of the matrix and analysis was performed. Further analysis by nuclear magnetic resonance (NMR) and electrospray ionization coupled with mass spectrometry (ESI-MS) showed that this matrix consists of at least four carbohydrates, namely glucosamine, arabinose, galactose and rhamnose. In the second part of the thesis, we studied the development of the Βotryococcus braunii microorganism and the isolation of a triterpene from a cyanobacterium. This microalgae is known to produce high amounts of lipids, but it has been found that its slow growth does not make it a key candidate for industrial exploitation. For this reason, a cyanobacterium growing much faster than this microalgae was used and it was possible to characterize and isolate a triterpene. The triterpene isolated from this cyanobacterium was squalene and was identified by gas chromatography coupled to mass spectrometry (GC-MS).

Language

Greek

Subject

Anabaena

Carbohydrates

Chlorella

EPS

Eukaryotic

GC-MS

Matrix

Microalgae

NMR