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| Identifier |
000454831 |
| Title |
The TuReLi TTX assay plate : Development of a Tunable and Reversible Linear Thermal Gradient Plate based on open-source modules for C. elegans thermotaxis assays |
| Alternative Title |
The TuReLi TTX assay plate |
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Author
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Σπινθάκη, Σοφία
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Thesis advisor
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Μπαζοπούλου, Δάφνη
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Reviewer
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Πανταζής, Αλέξανδρος
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| Abstract |
Thermal gradient platforms are used in a range of response testing applications from materials and
components characterization i.e., glass transmission T oC, melting point, MFFT, to biology research.
Thermotaxis (TTX) is the organism’s locomotion strategy in response to environmental temperature
changes or stimuli. This behavior ensures that organisms will locate themselves at optimal conditions
and is the subject of nervous system studies. In the popular model organism Caenorhabditis elegans,
TTX assays are employed for learning and memory studies i.e., how environmental signals shape
behavioral output, with regards to previous experiences. During those assays, C. elegans navigate
towards preferable isothermals when traveling up and down a thermal gradient. TTX assays require
technical components that provide sensitivity, stability, high-resolution and/or high throughput
capabilities. Commercially available equipment exists and requires minimum user expertise. However,
this type of equipment drastically increases the cost while limiting potential output, since it is usually
not compatible with other lab equipment. For that reason, research labs are turning into customized
platforms that are tailored to their in house setup but have no provision for implementation from other
users. Here, we developed a stand-alone, tunable TTX device: the TuReLi (Tunable, Reversible and
Linear) TTX assay plate, from 3D printed parts and readily available modules, which allows high
resolution multi-position temperature control and recording. Our system is open source-based,
affordable to fabricate and requires no industrial control units. Due to the flexible and user-friendly
operation, the TuReLi TTX is an ideal, universal solution for thermotaxis-based assays.
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| Language |
English |
| Issue date |
2023-04-05 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/a/0/2/metadata-dlib-1681902965-249564-27576.tkl
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| Views |
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