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Identifier |
000345752 |
Title |
Συμβολή της γενετικής και βιοτεχνολογίας στην κατανόηση της αποφρακτικής νόσου των αεραγωγών |
Author
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Ζερβού, Μαρία Ι.
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Thesis advisor
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Σιαφάκας, Νικόλαος
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Reviewer
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Γεωργόπουλος, Δημήτριος
Φρουδαράκης, Μάριος
Ζαννής, Βασίλειος
Μπούμπας, Δημήτριος
Καρδάσης, Δημήτριος
Τζανάκης, Νικόλαος
|
Abstract |
Previous studies have shown that microsatellite (MS) DNA instability (MSI) is detectable
in sputum cells in chronic obstructive pulmonary disease (COPD) and asthma. The aim of
the present study was to investigate whether asthma and COPD could be distinguished at
the MS DNA level. DNA was extracted from sputum cells and white blood cells from 63
COPD patients, 60 non-COPD smokers, 36 asthmatics and 30 healthy non-smokers. Ten
MS markers located on chromosomes 2p, 5q, 6p, 10q, 13q, 14q and 17q were analysed.
No MSI was detected in non-COPD smokers or healthy nonsmokers. A significantly
higher proportion of COPD patients exhibited MSI (49.2%) compared to asthmatics
(22.2%). MSI was detected even in the mild stages of COPD (33.3%) and asthma
(22.2%). No relationship was found between MSI and COPD severity. The most
frequently affected marker was D14S588 (17.5% in COPD and 2.7% in asthma). The
markers D6S344, G29802 and D13S71 showed alterations only in COPD, and G29802
was associated with a significantly decreased forced expiratory volume in one second
FEV1 (% predicted), whereas MSI in D6S344 was associated with a significantly higher
FEV1 (% pred). The frequency of microsatellite instability was higher in chronic
obstructive pulmonary disease than in asthma, and microsatellite instability in three
workers showed chronic obstructive pulmonary disease specificity. However, further
studies are needed to verify the differences between chronic obstructive pulmonary
disease and asthma at the microsatellite level.
|
Physical description |
148 σ. : πιν. ; 30 εκ. |
Language |
Greek |
Subject |
Pulmonary Disease, Chronic Obstructive |
|
Respiratory System |
Issue date |
2007-07-26 |
Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/6/1/1/metadata-dlib-babfbe412a90d124e02ba8970f9e0f62_1243410195.tkl
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Views |
283 |