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Identifier |
000364268 |
Title |
The possible involvement of mesenchymal stem cells (MSCs) in the pathophysiology of bone marrow (BM) failure associated with myelodysplastic syndromes (MDS) |
Alternative Title |
Διερεύνηση του πιθανού ρόλου των μεσεγχυματικών στελεχιαίων κυττάρων του μυελού των οστών στην παθογένεια των μυελοδυσπλαστικών σύνδρομων. |
Author
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Klaus, Mirjam
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Thesis advisor
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Παπαδάκη, Ελένη
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Reviewer
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Ηλιόπουλος, Αριστείδης
Σταθόπουλος, Ευστάθιος
Μπούμπας, Δημήτριος
Σαμώνης, Γεώργιος
Δημητρίου, Ελένη
Γουλιέλμος, Γεώργιος
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Abstract |
Defective hematopoiesis supporting capacity of bone marrow (BM) stroma has been
implicated in the pathophysiology of myelodysplastic syndromes (MDS).
The aim of this study is to explore whether the BM stroma progenitors, namely the
mesenchymal stem cells (MSCs), are primarily affected in MDS by evaluating the
reserves, the functional properties, as well as the cytogenetic characteristics, in
comparison to BM hematopoietic cells, in patients with de novo MDS ( n = 13). The
number, differentiation potential toward adipocytes/chondrocytes/osteoblasts and
immunosuppressive function in terms of inhibition of mitogen-induced T-cell
proliferation did not differ significantly between patient and normal ( n = 20) MSCs.
Patient MSCs did not show any aberrations in the production of proinflammatory or
growth-promoting cytokines and did not harbour the cytogenetic abnormalities
present in hematopoietic cells. Occasional patient and normal MSC cultures,
however, developed irrelevant chromosomal alterations (trisomies 5 and 7) with
uncertain pathophysiologic significance. Compared to controls, patient MSCs
displayed impaired proliferative and clonogenic potential through passages that
might represent a nonspecific abnormality associated with the chronic inflammatory
process present in patients’ BM.
These data suggest that BM MSCs from MDS patients do not belong to the abnormal
clone and do not represent the main cellular source contributing to the inflammatory
marrow microenvironment.
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Language |
Greek |
Subject |
Bone marrow diseases |
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Hematology |
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Hemic and lymphatic systems |
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Αιματικό και λεμφικό σύστημα |
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Αιματολογία |
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Νόσοι μυελού των οστών |
Issue date |
2010-03-23 |
Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/4/c/f/metadata-dlib-487b889491971f0c2dae5073078edf4a_1298534037.tkl
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Views |
229 |