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Identifier

000364268

Title

The possible involvement of mesenchymal stem cells (MSCs) in the pathophysiology of bone marrow (BM) failure associated with myelodysplastic syndromes (MDS)

Alternative Title

Διερεύνηση του πιθανού ρόλου των μεσεγχυματικών στελεχιαίων κυττάρων του μυελού των οστών στην παθογένεια των μυελοδυσπλαστικών σύνδρομων.

Author

Klaus, Mirjam

Thesis advisor

Παπαδάκη, Ελένη

Reviewer

Ηλιόπουλος, Αριστείδης
Σταθόπουλος, Ευστάθιος
Μπούμπας, Δημήτριος
Σαμώνης, Γεώργιος
Δημητρίου, Ελένη
Γουλιέλμος, Γεώργιος

Abstract

Defective hematopoiesis supporting capacity of bone marrow (BM) stroma has been implicated in the pathophysiology of myelodysplastic syndromes (MDS). The aim of this study is to explore whether the BM stroma progenitors, namely the mesenchymal stem cells (MSCs), are primarily affected in MDS by evaluating the reserves, the functional properties, as well as the cytogenetic characteristics, in comparison to BM hematopoietic cells, in patients with de novo MDS ( n = 13). The number, differentiation potential toward adipocytes/chondrocytes/osteoblasts and immunosuppressive function in terms of inhibition of mitogen-induced T-cell proliferation did not differ significantly between patient and normal ( n = 20) MSCs. Patient MSCs did not show any aberrations in the production of proinflammatory or growth-promoting cytokines and did not harbour the cytogenetic abnormalities present in hematopoietic cells. Occasional patient and normal MSC cultures, however, developed irrelevant chromosomal alterations (trisomies 5 and 7) with uncertain pathophysiologic significance. Compared to controls, patient MSCs displayed impaired proliferative and clonogenic potential through passages that might represent a nonspecific abnormality associated with the chronic inflammatory process present in patients’ BM. These data suggest that BM MSCs from MDS patients do not belong to the abnormal clone and do not represent the main cellular source contributing to the inflammatory marrow microenvironment.

Language

Greek

Subject

Bone marrow diseases

Hematology

Hemic and lymphatic systems

Αιματικό και λεμφικό σύστημα

Αιματολογία

Νόσοι μυελού των οστών

Issue date

2010-03-23

Collection