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| Identifier |
39544 |
| Title |
Η επίδραση της υπεριώδους ακτινοβολίας στα δερματικά κύτταρα και η χρήση της πολυφασματικής απεικόνισης για τη μελέτη των δερματικών λοιμώξεων |
| Creator |
Kosmadaki, Maria G
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| Abstract |
Ultraviolet (UV) irradiation was used in the current thesis as a tool for the study of the induction of Vascular Endothelial Growth Factor (VEGF). VEGF increases vascular permeability, induces proliferation of vascular endothelial cells and is broadly recognized as a potent angiogenic factor. It is known that UV irradiation increases Tumor Necrosis Factor alpha (TNF-α) in cultured keratinocytes and that TNF-α upregulates VEGF. We investigated if blocking of TNF-α affected the expression, synthesis and secretion of VEGF after exposure of keratinocytes to UV irradiation. Indeed, exposure of keratinocytes to 30 mJ/cm2 of UVB, resulted in an increase of VEGF mRNA, protein synthesis and protein secretion. Furthermore, treatment of cells with TNF-α resulted in an induction of VEGF in a dose-dependent manner. Addition of a TNF-α-blocking peptide in the supernatant abrogated the TNF-α-induced upregulation of VEGF but had no effect in the upregulation of VEGF after UV irradiation. Our results indicate that the UV-induced upregulation of VEGF is probably controlled by multiple mechanisms and blocking only one pathway does not abrogate the upregulation of VEGF. Furthermore, via advanced biotechnology (Multi-Spectral Imaging System), UV irradiation was used to excite and evaluate fluorescence in the diagnosis and treatment of skin infections. Five patients with erythrasma were exposed to UV irradiation to detect the fluorescence on erythrasma lesions, before and during topical treatment. At the same times, bacterial cultures were obtained from the lesions. Corynebacterium minutissimum was isolated and cultures were exposed to UV irradiation to check and measure the fluorescence of the microorganisms every 24 hours. Fluorescence was detected on erythrasma lesions for different intervals in different patients, from 2 to 10 days. At the extinction of fluorescence, improvement but no cure of the skin lesions was observed and C. minutissimum was still isolated in cultures. The fluorescence of the cultured C. minutissimum decayed with time till final cessation at different intervals. Recultures of non-fluorescent cultures gave new cultures that fluoresced as the mother cultures did. We conclude, that the coral-red fluorescence of C. minutissimum may be used as a reliable diagnostic criterion for erythrasma but is not indicative of disease cure nor it is directly associated with the organisms viability. In addition, ten patients with tinea versicolor were examined under UV irradiation for yellow fluorescence. Only three of them presented with the characteristic yellow fluorescence of the disease. Fluorescence on tinea versicolor lesions may not consist a reliable diagnostic criterion, since only three of the ten patients examined exhibited the above mentioned yellow fluorescence when excited with UV irradiation.
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| Language |
Greek |
| Issue date |
2004-03-01 |
| Date available |
2004-08-26 |
| Collection
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School/Department--School of Medicine--Department of Medicine--Doctoral theses
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Type of Work--Doctoral theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/3/a/c/metadata-dlib-2004kosmadaki.tkl
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| Views |
200 |
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