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Identifier 000419715
Title Μελέτη της γενετικής βάσης της ανθεκτικότητας & του κόστους προσαρμοστικότητας στο έντομο Tuta absoluta για το εντομοκτόνο indoxacarb
Alternative Title Study of the genetic base of resistance and fitness cost in T. absoluta for indoxacarb
Author Μετοχιανάκη, Κατερίνα Δ.
Thesis advisor Βόντας, Ιωάννης
Reviewer Καλαντίδης, Κρίτων
Ροδιτάκης, Εμμανουήλ
Abstract The tomato borer Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is considered as the most important pest for tomato crops. DPX-MP062 (indoxacarb) is an oxidiazine insecticide which has activity against Lepidoptera. Extensive utilization of indoxacarb pesticide against insect Τ. absoluta causes the development of resistance in field populations of this lepidopteran pest. To evaluate the risk of resistance evolution and to develop a better resistance management strategy, a field-collected population was selected with indoxacarb in the laboratory. Thereafter, the inheritance and fitness cost of indoxacarb resistance in T. absoluta were investigated. In this study the genetic basis of oxidiazine resistance was investigated in an oxidiazine resistant strain from Ierapetra. The genetics of resistance was investigated by reciprocally crossing strain Ierapetra with a susceptible strain from Peloponnhso and revealed an autosomal incompletely recessive mode of inheritance. x² value and degrees of freedom revealed that multiple genes are responsible for the resistant. Fitness cost of the resistant strain from Ierapetra was also studied. Specifically, from the study of female and male adults viability, the duration of developmental stages (egg, L1, L2, L3, L4, pupa), the longevity, the weight of male and female pupa, the mortality in every developmental stage, fertility, parameters as net reproduction rate (Rₒ), gross reproductive rate (GRR), mean generation time (Τ), intrinsic rate of increase (r) were identified and compared with the susceptible strain. No differentiation between susceptible and resistant strain in indoxacarb was revealed. Additional, reverse resistance was detected to the population from Ierapetra. Higher total mortality in whole developmental stages resistant strain may be responsible for this reverse resistance.
Language Greek
Issue date 2018-11-23
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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