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Identifier 000419587
Title Συγκριτική μελέτη λοιμογονικότητας του βακτηριακού παθογόνου ιχθύων, Aeromonas veronii bv sobria. Γενετική βάση και in vivo μελέτη
Alternative Title Comparative study of the virulence of the bacterial fish pathogen, Aeromonas veronii bv sobria. Genetic basis and in vivo study
Author Τρίγκα, Ανδριάνα Α.
Thesis advisor Παυλίδης, Μιχάλης
Reviewer Καθαρός, Παντελής
Καλαντίδης, Κρίτων
Abstract European seabass, Dicentrarchus labrax is one of the most important species for the Mediterranean Aquaculture. The past years, morbidity and mortality of cage-cultured seabass due to infections by Aeromonas veronii bv sobria have been reported from Greece and Turkey which are the main producers of the species. More than 50 strains of the pathogen have been isolated from various locations in Greece and Turkey and have been partially characterized. The genomes of nine strains have been fully sequenced. These strains are representatives of the geographic origin of isolation but also of the various phenotypes of the bacteria since isolates have differences in motility and pigment production. Virulence of the sequenced strains was examined in adult zebrafish where LD50-24h values ranged between 4.3 x 105 – 1.3 x 106 cfu/ fish following intraperitoneal administration. Differences in the phenotypes and virulence of the strains were studied through comparative genomic analysis. Two of the sequenced strains were tested in adult seabass, in which virulence was significant higher. Following a 2.5h immersion challenge in 105 cfu/ ml, seabass suffered mortality of 100% within 10 days post challenge. The main virulence factors were investigated and multiple genes were identified, some of which are associated with the pathogenicity of Gram negative bacteria. Such factors include the secretion systems (type II, III, VI), polar and lateteral flagella, aerolysins, hemolysins and type IV pili. The nine strains contain the same repertoire of virulence genes and some of them, such as the important type III secretion system, are found in specific loci on the genomes called Genomic Islands that are indicative of horizontal gene transfer.
Language Greek
Subject Dicentrarchus labrax
Sea bass
Secretion system
Virulence factors
Whole-genome sequencing
Εκκριτικό σύστημα
Λοιμογονικοί παράγοντες
Πλήρης αλληλούχιση γονιδιώματος
Issue date 2018-11-23
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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