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Identifier

000446833

Title

Ανάλυση του ρόλου του SERRATE σε συνθήκες βιοτικής καταπόνησης από το ιοειδές PSTVd σε φυτά Nicotiana benthamiana

Alternative Title

Analysis of the role of SERRATE under biotic stress conditions induced by PSTVd voroid in Nicotiana benthamiana plants/

Author

Γεμελιάρη, Πετρούλα Μ.

Thesis advisor

Καλαντίδης, Κρίτων

Abstract

RNA silencing was originally a defense mechanism, mainly antiviral, which was present in the last common ancestor of all eukaryotic organisms and provided protection against RNA invaders in the cytoplasm. Over the years and the evolutionary pressure that was exerted on organisms, this mechanism acquired a regulatory role for the plant genome. In terms of the operation of the mechanism, it is the most conserved mechanism in eukaryotic organisms till today. Plant organisms have 3 main silencing pathways and one of them is the miRNA pathway. The miRNA silencing pathway has been further studied in terms of its role as a developmental regulator. Dicer Like 1 (DCL1) is the most important factor in the miRNA silencing pathway, but due to its dominant role in plant growth and viability, for the study of this pathway the research focuses on the equally important protein, SERRATE (SE). This protein was first identified in 1964 as a mutant of the Arabidopsis thaliana plant by Rédei and Hirono and was later identified as a key regulator of the miRNA silencing pathway. Under conditions of reduced expression, a reduced accumulation of both pre-miRNAs and mature double-stranded miRNAs is observed. The SE protein has also been found to be involved in other processes, most importantly the splicing and alternative splicing process of genes. Previous studies have focused on the endogenous mechanism of RNA silencing with the effect of specific miRNAs on the control or dominance of the pathogen, with an emphasis mainly on viral and bacterial infections. At the same time, a positive correlation has been found between the infectivity of viruses of the Pospiviroidae family and the expression levels of the SERRATE gene, in experiments with plant that show repression of this gene. In this study, an attempt will be made to further understand the role of the endogenous pathway of silence in the response of plants to conditions of biotic stress. Nicotiana benthamiana plants with a disfunctional miRNA pathway will be used for this purpose. The transcription levels of the SERRATE gene in infected and non-infected plants will be studied. In addition, in the 5 'untranslated region of the SERRATE gene promoter there are 2 extra ATGs. Here, we try to study possible effect of these ATGs in SERRATE translation both under or not biotic stress conditions, in wild type and transgenic plant.

Language

Greek

Subject

MicroRNAs biogenesis pathway

Transcription

Βιοτική καταπόνηση

Μεταγραφή

Μονοπάτι βιογένεσης των MicroRNAs

Issue date

2022-03-28

Collection

School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses