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Identifier |
000446833 |
Title |
Ανάλυση του ρόλου του SERRATE σε συνθήκες βιοτικής καταπόνησης από το ιοειδές PSTVd σε φυτά Nicotiana benthamiana |
Alternative Title |
Analysis of the role of SERRATE under biotic stress conditions induced by PSTVd voroid in Nicotiana benthamiana plants/ |
Author
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Γεμελιάρη, Πετρούλα Μ.
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Thesis advisor
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Καλαντίδης, Κρίτων
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Abstract |
RNA silencing was originally a defense mechanism, mainly antiviral, which was
present in the last common ancestor of all eukaryotic organisms and provided protection
against RNA invaders in the cytoplasm.
Over the years and the evolutionary pressure that was exerted on organisms, this
mechanism acquired a regulatory role for the plant genome. In terms of the operation
of the mechanism, it is the most conserved mechanism in eukaryotic organisms till
today. Plant organisms have 3 main silencing pathways and one of them is the miRNA
pathway. The miRNA silencing pathway has been further studied in terms of its role as
a developmental regulator.
Dicer Like 1 (DCL1) is the most important factor in the miRNA silencing pathway, but
due to its dominant role in plant growth and viability, for the study of this pathway the
research focuses on the equally important protein, SERRATE (SE). This protein was
first identified in 1964 as a mutant of the Arabidopsis thaliana plant by Rédei and
Hirono and was later identified as a key regulator of the miRNA silencing pathway.
Under conditions of reduced expression, a reduced accumulation of both pre-miRNAs
and mature double-stranded miRNAs is observed. The SE protein has also been found
to be involved in other processes, most importantly the splicing and alternative splicing
process of genes.
Previous studies have focused on the endogenous mechanism of RNA silencing with
the effect of specific miRNAs on the control or dominance of the pathogen, with an
emphasis mainly on viral and bacterial infections. At the same time, a positive
correlation has been found between the infectivity of viruses of the Pospiviroidae
family and the expression levels of the SERRATE gene, in experiments with plant that
show repression of this gene.
In this study, an attempt will be made to further understand the role of the endogenous
pathway of silence in the response of plants to conditions of biotic stress. Nicotiana
benthamiana plants with a disfunctional miRNA pathway will be used for this purpose.
The transcription levels of the SERRATE gene in infected and non-infected plants will
be studied. In addition, in the 5 'untranslated region of the SERRATE gene promoter
there are 2 extra ATGs. Here, we try to study possible effect of these ATGs in
SERRATE translation both under or not biotic stress conditions, in wild type and
transgenic plant.
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Language |
Greek |
Subject |
MicroRNAs biogenesis pathway |
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Transcription |
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Βιοτική καταπόνηση |
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Μεταγραφή |
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Μονοπάτι βιογένεσης των MicroRNAs |
Issue date |
2022-03-28 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/c/c/c/metadata-dlib-1647861338-270313-23512.tkl
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Views |
365 |