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Identifier 000421787
Title Μελέτη του ρόλου του Serrate στην απόκριση των φυτών σε βιοτικές καταπονήσεις στα φυτά N. benthamiana & N. tabacum
Alternative Title Studying the role of Serrate in biotic stress response in N. benthamiana & N. tabacum plants
Author Κρυοβρυσανάκη, Νικολέτα
Thesis advisor Καλαντίδης, Κρίτων
Reviewer Τσαγρή, Ευθυμία
Βόντας, Ιωάννης
Κοτζαμπάσης, Κυριάκος
Μόσχου, Παναγιώτης
Μαλιόγκα, Βαρβάρα
Σαρρής, Παναγιώτης
Abstract RNA silencing has a prominent role in all eukaryotic organisms as a mechanism for regulating gene epression as well as regulating the relationship between plant - pathogens (ανασκοπήσεις, Huang et al., 2016; Ruiz-Ferrer and Voinnet, 2009). Especially, with regard to the study of the mechanism correlated with biotic stress, a number of studies have been carried out, both by us and others (Blevins et al., 2006; Dadami et al., 2013; Gomez et al., 2008; Katsarou, 2018; Katsarou et al., 2016; Minoia et al., 2014; Torchetti et al., 2016). They deal mainly with the study of the exogenous silencing pathway post virus and viroid infections and the mechanisms governing their relations. There have been identified factors that have a positive role in plant defense but also factors that have a positive effect on pathogen prevalence. Similar studies have been conducted also around the edogenous mechanism of RNA silencing and are related to the effect of specific miRNAs on the control or dominance of the pathogen, focusing mainly on viral and bacterial infections (ανασκόπηση, Liu et al., 2017; Navarro et al., 2006; Niu et al., 2016; Zhang et al., 2011). In the case of viroids in particular, the interest is focused on the fact that viroids themselves, due to their structural characteristics, seem to act as pri-miRNA and the produced vdsiRNAs, may function similar to miRNAs, regulating the expression of endogenous genes and thus defining the ideal conditions for their prevalence (Landry and Perreault, 2005; Markarian et al., 2004; Wang et al., 2004). It is even more interesting to refer to viroids as they have the specific capacity while inducing and are targeted by RNA silencing (Itaya et al., 2001; Papaefthimiou et al., 2001; Tabler and Tsagris, 2004) however, they manage to escape their own degradation, possibly due to their secondary structure (Itaya et al., 2007) or due to their location (Qi and Ding, 2003). By combining the information already available, we wanted to study more closely the correlation between the miRNA pathway and biotic stress. The biotic stress factore used were mainly viroids (Potato spindle tuber viroid, PSTVd and Hop stunt viroid, HSVd) and secondary a virus (Cucumber mosaic virus, CMV) and a bacterium (Pseudomonas syringae, P.syringae). For this purpose, plants with reduced functionality of the miRNA pathway were used and their effect on the infectivity of the above pathogens was studied. From a previous work in our lab, we have already generated plants, through RNAi, that suppress the primary regulator of the miRNA pathway, Dicer-like 1 (DCL1) (Dadami et al., 2013). New infections with PSTVd were made in DCL1i plants and the results were taken into account with the previous ones (Dadami et al., 2013). However, we have been unable to generate viable plants with very strong DCL1 suppression, we have generated transgenic N.benthamiana and N.tabacum plants, which 8 suppress an equally important regulator of the miRNA pathway, the SERRATE (SE) gene. Once functional SE suppressive lines of both plant species have been characterized, we performed infectivity experiments with PSTVd, HSVd, CMV and P.syringae plant pathogens. Regarding PSTVd, both the effect of reduced SE expression on its infectivity and the effect of viroid on SE expression were studied. It was found that there is a particularly strong positive correlation between SE and PSTVd, which in conditions of strong SE suppression may even fail to infect the plants. There were indications for less intense positive correlation regarding the effect of SE on CMV infectivity, while till now, the infectivity of the bacterium P.syringae is negatively affected by SE. However, additional experimental procedures are required to confirm the results in the case of viruses and bacteria infectivity in SE suppressed conditions.
Language Greek
Subject Biotic stress
RNA silencing
RNA σίγηση
Βιοτική καταπόνηση
Μικρά RNA
Issue date 2019-04-04
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Doctoral theses
  Type of Work--Doctoral theses
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