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Identifier 000419566
Title Κατασκευή τρισδιάστατων ικριωμάτων μέσω πολυφωτονικού πολυμερισμού για εφαρμογές στην αναγεννητική ιατρική και μηχανική ιστών
Alternative Title 3D structuring via multiphoton polymerization for tissue engineering and regenerative medicine
Author Παρκατζίδης, Κωνσταντίνος Ζ.
Thesis advisor Στρατάκης, Εμμανουήλ
Βαμβακάκη, Μαρία
Abstract Multiphoton lithography, based on photopolymerization, is a powerful technique for the fabrication of 3D structures used in tissue engineering, regenerative medicine, and other biomedical applications. Multiphoton polymerization is based on the localized polymerization / cross-linking of photosensitive materials induced by femtosecond laser pulses. The use of a suitable photoinitiator is required to initiate the polymerization / cross-linking process and attain the 3D structures. In the current Master Thesis, we have developed materials and processes suitable for multiphoton polymerization to fabricate 3D porous scaffolds for tissue engineering and regenerative medicine applications. In Chapter 2, the modification of gelatin, which is a well known biopolymer, with a methacrylic anhydride in order to introduce photopolymerizable moieties of the methacrylamide group is presented. In addition, we have investigated the suitability of this gelatin methacrylamide biopolymer in the fabrication 3D scaffolds in the absence of any photoinitiator. Such an approach is highly desirable in biological application, due to the elimination of the toxic photoinitiator molecules, as well as the highly diffusive free radicals which are produced by these molecules. The biocompatibility of the synthesized material, both in the form of 2D films as well as in 3D porous scaffolds, was examined via biocompatibility assays of live/dead cells as well as via immunocytochemistry for actin assay using NIH 3T3 fibroblast cells. In Chapter 3, we have synthesized gelatin methacrylamide and a photopolymerizable water soluble chitosan derivative. Hybrid materials of these two biopolymers were used to fabricate 3D scaffolds for cell culture application.. Near IR laser irradiation, operated at 800nm, was employed to fabricate 3D scaffolds, in the presence of eosin-Y, as a water soluble, FDA-approved and biocompatible photoinitiator, in the absence of any other co-initiators or co-monomers reported in the literature. We saggasted that the free amine groups of GelMA and the chitosan derivative act as co-initiator 4 moieties ans support the photopolymerization process. Cell behavior on the hybrid materials on 2d films as well as 3D scaffolds was examined, using primary dental pulm stem cells, which showed excellent cell adhesion, growth and proliferation verifying the biocompatibility of this hybrid material. In the last Chapter, dual functional 3D scaffolds, for tissue engineering and regenerative medicine were developed. We employed a hybrid organic-inorganic material, functionalized with thymol methacrylate moieties as a biocompatible and antimicrobial material for tissue engineering applocations. Thymol, as a natural product derived from essential oils of the plants, has great antimicrobial activity. 3D scaffolds of material developed showed excellent biocompatibility and simultaneously strong antimicrobial action. Biological studies using primary dental pulm stem cells showed an increased cell adhesion and proliferation on the 3D scaffolds whereas their antifouling behavior was confirmed by e-coli bacteria culture in prolonged of microbial assays.
Language English
Issue date 2019-03-27
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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