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Identifier 000417164
Title Μελέτη των CIS ρυθμιστικών στοιχείων του γονιδίου Hey της Δροσόφιλας melanogaster
Alternative Title Study of the CIS-regulatory modules of Drosophila melanogaster Hey gene
Author Πιτσιδιανάκη, Ιωάννα Γ.
Thesis advisor Μοναστιριώτη, Μαρία
Reviewer Δελιδάκης, Χρήστος
Καρδάσης, Δημήτριος
Abstract Hey gene encodes a basic-Helix-Loop-Helix transcription factor transiently expressed in the newborn neurons of Drosophila melanogaster central nervous system. It has been shown that Hey expression is Notch dependent in some regions, while in others it is Notch independent. In addition, homozygous Hey deletion is late embryonic lethal and the rare escapers die as 1st or 2nd instar larvae. Bioinformatics analysis revealed two potential enhancer elements for Hey, containing suppressor of Hairless binding sites, one region is located in the second intron of the gene sequence and the other upstream of the promoter region. In the present work we provide a mutation analysis for both enhancer elements, by generating transgenic reporter fly lines, in an attempt to dissect the functional role of each binding site of these two regulatory modules. Furthermore, we attempted deleting the region of the intronic enhancer in the endogenous sequence of Drosophila melanogaster Hey gene in order to decipher the importance or a potential redundancy of the two enhancers, without successful results. Concluding, the immunohistochemistry analysis of the transgenic reporter fly lines provide robust proof of the regulatory role of the fragments of Hey enhancer elements. The present study contributes to a better understanding of Notch signaling in the context of Hey, a necessary for viability of Drosophila melanogaster, gene.
Language English
Issue date 2018-07-20
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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