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Identifier 000419869
Title Laser microfabrication of porous collagen scaffolds for biological applications
Alternative Title Μικροκατεργασία πορώδων ικριωμάτων κολλαγόνου με χρήση υπέρστενων παλμών λέιζερ για τον έλεγχο κυτταρικών λειτουργιών
Author Γιαννάκου, Χριστίνα
Thesis advisor Γραβάνης, Αχιλλέας
Στρατάκης, Εμμανουήλ
Reviewer Σιδηροπούλου, Κυριακή
Τζεράνης, Δημήτριος
Abstract A basic part of bioengineering is the materials. Materials are what enable us to cultivate and control cells various functions. This has two basic applications, Regenerative medicine and 3D culture. The goal of this field is to find treatments for some injuries or organs that have suffered some damage. According to the literature, materials seem to have helped in the accomplishment of this. What interests us in this work is to make 3D scaffolds - tissue models and understand how the cell's environment affects its function. There is a wide range of materials, but we are interested in Porous Scaffolds based on Collagen. It has a biological activity and low antigenicity. Because of its pores, cells enter and bind to the material. The way they interact with the environment affects their behavior. The environment affects the way of their outgrowing, orientation, organization and interactions with each other. Also affects the way they interact with other type of cells. Collagen based scaffolds have some limitations in size and construction. Here's where the lasers come, which allow us to make structures with high precision, complex shapes and in the scale of micrometers. In this way we can build structures and shapes and control how the environment that perceives the cell affects it. Specifically the aim of the present work is the creation of 2D and 3D structures, their characterization and in vitro experiments with motor neuron and muscle cells. At the level of structures the characterization can be done with SEM images, mechanical collagen resistance and, SHG microscopy. Then in the in vitro experiments we will construct such structures that will study the response of co culture of motor neuron and muscle cells. The goal, ‘’the dream’’ is to be able to build structures with direct application to clinical regenerative medicine and in vivo trials. A field in which we have to overcome a lot of but one step at a time.
Language English
Subject Κυτταρικές καλλιέργειες
Issue date 2018-12-05
Collection   Faculty/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
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