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Title

Αξιοποίηση των αποβλήτων τυροκομείων και παραγωγή βιομάζας υψηλής διατροφικής αξίας με χρήση του μικροφύκους Chlorella sp.

Alternative Title

Valorization of cheese-whey wastewaters and production of high-nutritional value biomass using the green microalga Chlorella sp.

Author

Τσολακίδη, Αγγελική

Thesis advisor

Γανωτάκης, Δημήτριος

Abstract

In the present thesis, a methodology for Chlorella biomass production using primary and secondary cheese whey wastewaters, was investigated. This cheese whey was originated from sheep and goat milk. Results of this study proved that cheese whey waste-waters have the potential to be used as substrates in culture media for microalgae using the appropriate experimental approach. Initial experiments were performed in small culture volume. In the primary fraction (rich in nutrients), microalgae exhibited high biomass production and photosynthetic efficiency. In the secondary fraction, growth was limited by the high salinity of the wastewaters and cell growth was only observed in diluted cheese whey wastewaters. For this purpose, these fractions were mixed together in various volumetric ratios, in order to decrease the salt content of the secondary. This approach led to an increase in both biomass production and photosynthetic efficiency to comparable levels with control cultures. In the last stage, microalgal growth was studied in pilot laboratory scale bioreactors (1000 mL volume). Cultivation in higher volumes presented comparable results to those of initial experiments. The dry biomass collected had been enriched in protein and polyunsaturated fatty acids content. In all cases the only pre-treatment performed was the autoclaving of the waste-waters and the addition of a trace mineral solution. The outcomes of the present thesis clearly show that cheese whey waste-waters have the potential to be used as a proper nutrient substrate for the cultivation of microalgae with their simultaneous bioremediation and high-value biomass production.

Language

Greek

Subject

Growth

Photosynthesis

Primary cheese whey,

Secondary cheese whey

Ανάπτυξη

Απόβλητα τυροκομείων

Βιομάζα

Φωτοσύνθεση

Issue date