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Title

Μελέτη της βιοαποκατάστασης αποβλήτων τυροκομείων υψηλής αλατότητας με χρήση του πράσινου μικροφύκους Chlorella sp

Alternative Title

A study of the bioremediation of high-salinity cheese-whey wastewaters by the green microalga Chlorella sp

Author

Βισκαδουράκη, Ευγενία-Στεφανία

Thesis advisor

Γανωτάκης, Δημήτριος

Abstract

The aim of this research was the rational design of cheese-whey based media for Chlorella biomass production, following up previous research work of our research group. The experiments focused on the elimination of unnecessary pretreatment steps, as well as improvement of the biomass production yield. Previous studies of our research group have shown that microalgae of the Chlorella genus can be grown in whey-based media with the simple addition of trace elements. Given that milk (and consequently whey) contains some trace metals, the addition of trace metals in various combinations was studied, in order to find the essential elements that are not present in whey. Furthermore, the effect of the initial cell concentration on the growth of Chlorella in primary and secondary cheese whey was studied. An advantage of the developed methodology is that this microalga is able to achieve growth in whey, even at very low initial cell concentration levels. In order to improve growth rates and biomass production yields, the effect of increasing the initial microalgal cell concentration was studied as well. Our results have shown that the addition of both ferrous and manganese (II) ions is the minimum requirement for the growth of Chlorella cells in cheese whey. In addition, cells grown in culture media based on primary cheese whey exhibited higher growth compared to the respective ones in secondary cheese whey. Increasing the initial cell concentration resulted in pronounced growth rates, but the final biomass produced remained the same. The results of this thesis showed that the optimization of our methodology was achieved, and these findings can be a tool to reduce the cost of large-scale applications.

Language

Greek

Subject

Bioremediation

Dairy wastewaters

Growth

Initial cell concentration

Microalgae

Photosynthesis

Trace minerals

Ανάπτυξη

Βιοαποκατάσταση