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Identifier 000412869
Title Μέρος 1ο : Παραγωγή τερπενοειδών υψηλής εμπορικής αξίας με μεθόδους ιστοκαλλιέργειας σε φυτά Nicotiana benthamiana Μέρος 2ο : Παραγωγή πρωτεϊνών υψηλής εμπορικής αξίας με τη μέθοδο του Αγροεμποτισμού σε φυτά Nicotiana benthamiana
Alternative Title Production of high value proteins in Nicotiana benthamiana plants through agroinfiltration
Author Κάτσαβου, Ευαγγελία Α.
Thesis advisor Καλαντίδης, Κρίτων
Abstract Plants have emerged as a promising alternative expression system for production of high value proteins because they offer several potential advantages, including low production costs, ease of scale-up to commercial quantities of production and reduced risk of product contamination by mammalian viruses or toxins. Τerpenes have properties as chemicals in food, cosmetics, pharmaceutical and biotechnology industries, with great economical importance. The DXS (1-deoxyxylulose 5- phosphate synthase) enzyme catalyzes the first step of the MEP pathway for terpene production in plants. That step is rate limitimg, underlying the importance of the DXS enzyme for the production of terpenes. In the first part of this thesis the DXS (isolated from the Cistus creticus plant) which is cloned in an overexpression vector, is used for Agrobacterium transformation. Nicotiana benthamiana leaf discs are transformed and four independent plant lines that bring one copy of the transgene are generated through tissue culture. Additionally, plants that have a Virp1 (bromodomain-containing protein that interacts with PSTVd) knock out are generated using the system of sgRNAs. PNGaseA (Peptide:N-glycanase) is a deglycosylation enzyme, of major economical importance for the study of the glycome. In the second part of this thesis, the PNGaseA gene is isolated from almond seeds, cloned in proper overexpression vector and expressed transiently through Agroinfiltration in Nicotiana benthamiana plants. The gene is successfully isolated and transcriptionally active.
Language Greek
Subject DXS
Issue date 2017-11-22
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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