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Identifier

000443256

Title

Δημιουργία γενετικά τροποποιημένων στελεχών του βακτηρίου Pseudomonas. sp phDV1 για βελτιστοποίηση της παραγωγής πολύ-(3-υδρόξυ-βουτυρικού)- εστέρα (PHB)

Alternative Title

Generation of genetically modified strains of Pseudomonas sp. phDV1 to optimize the production of poly-(3- hydroxy-butyrate)-ester (PHB)

Author

Δρακωνάκη, Αθηνά Ι.

Thesis advisor

Τσιώτης, Γεώργιος

Reviewer

Γανωτάκης, Δημήτριος
Παυλίδης, Ιωάννης

Abstract

Environmental pollution by plastics is one of the main impacts of man's presence on the planet. Various solutions have been proposed to address the problem, including the replacement of conventional plastics with polymers produced by microorganisms. One of the most studied categories of bioplastics are poly-hydroxyalkanoates (PHAs). The most studied polymer belonging to this category is the polyhydroxy-butyrate (PHB). It is produced by several microorganisms and some plants under stress conditions. Pseudomonas sp. phDV1 is a gram-negative bacterium, which has the ability to produce PHB. The polymer in the cells is organised into a structure known as carbosome. In this structure there are proteins associated with its formation, synthesis and catabolism. These enzymes are: a synthase (phaC), a depolymerase (phaZ), phasins (phaP) and a protein regulator (phaR). The synthase and the depolymerase are associated with the synthesis and degradation of PHB, while phasins have a catalytic role in the formation of the polymer. The protein regulator controls the expression of phasins. Several studies that have been carried out have aimed at improving the production of PHB in various microorganisms using various molecular biology techniques. The thesis focuses on enhancing polymer production by deletion of phaR and phaZ genes in the specific strain. The mutations created, ΔphaR and ΔphaZ, were studied and compared with WT.

Language

Greek

Subject

Homologous recombination

ΔphaR

ΔphaZ

Ομόλογος ανασυνδυασμός

Issue date

2021-11-24

Collection

School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses

Type of Work--Post-graduate theses