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Identifier |
000443256 |
Title |
Δημιουργία γενετικά τροποποιημένων στελεχών του βακτηρίου Pseudomonas. sp phDV1 για βελτιστοποίηση της παραγωγής πολύ-(3-υδρόξυ-βουτυρικού)- εστέρα (PHB) |
Alternative Title |
Generation of genetically modified strains of Pseudomonas sp. phDV1 to optimize the production of poly-(3- hydroxy-butyrate)-ester (PHB) |
Author
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Δρακωνάκη, Αθηνά Ι.
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Thesis advisor
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Τσιώτης, Γεώργιος
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Reviewer
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Γανωτάκης, Δημήτριος
Παυλίδης, Ιωάννης
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Abstract |
Environmental pollution by plastics is one of the main impacts of man's presence on
the planet. Various solutions have been proposed to address the problem, including
the replacement of conventional plastics with polymers produced by microorganisms. One of the most studied categories of bioplastics are poly-hydroxyalkanoates (PHAs). The most studied polymer belonging to this category is the polyhydroxy-butyrate (PHB). It is produced by several microorganisms and some plants
under stress conditions.
Pseudomonas sp. phDV1 is a gram-negative bacterium, which has the ability to
produce PHB. The polymer in the cells is organised into a structure known as
carbosome. In this structure there are proteins associated with its formation,
synthesis and catabolism. These enzymes are: a synthase (phaC), a depolymerase
(phaZ), phasins (phaP) and a protein regulator (phaR). The synthase and the
depolymerase are associated with the synthesis and degradation of PHB, while
phasins have a catalytic role in the formation of the polymer. The protein regulator
controls the expression of phasins.
Several studies that have been carried out have aimed at improving the production of
PHB in various microorganisms using various molecular biology techniques. The
thesis focuses on enhancing polymer production by deletion of phaR and phaZ
genes in the specific strain. The mutations created, ΔphaR and ΔphaZ, were studied
and compared with WT.
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Language |
Greek |
Subject |
Homologous recombination |
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ΔphaR |
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ΔphaZ |
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Ομόλογος ανασυνδυασμός |
Issue date |
2021-11-24 |
Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/2/b/8/metadata-dlib-1635766984-614935-16752.tkl
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Views |
274 |