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Identifier 000443249
Title Ανάπτυξη και εφαρμογή πηγών ιοντισμού υπερηχητικής εκνέφωσης με φασματομετρία μάζας για την ανάλυση περιβαλλοντικών και βιολογικών δειγμάτων
Alternative Title Development and application of sonic spray ionization sources for mass spectrometric analysis of environmental and biological samples
Author Γραφανάκη, Σοφία Ν.
Thesis advisor Περγαντής, Σπυρίδων
Reviewer Απόστολος, Σπύρος
Λυδάκης-Σημαντήρης, Νικόλαος
Abstract The technique of Sonic Spray Ionization Mass Spectrometry (SSI-MS) was the basis of the development and application of two analytical methods, Orthogonal Sonic spray Ionization (Ortho-SSI) and Desorption sonic spray Ionization Mass Spectrometry (DeSSI-MS). The development of each ionization source involves the selection of optimum parameters before the analysis of real samples. The first part consists of the detailed development of the Orthogonal Sonic spray Ionization source. That involves the interaction of a high velocity spray with a cylindrical rode that bends the spray from a SSI nebulizer and thus introduces SSI – originating droplets into the MS in order to improve the efficiency with which high performance liquid chromatography (HPLC) can be coupled to SSI-MS. The HPLCOrtho-SSI-MS/MS was used to separate and detect chlorate (ClO3-), perchlorate (ClO4-) and bromate (BrO3-) ions and quantitate these anions in water samples. In the second part, Chlorfenapyr insecticide metabolism was investigated for four recombinant proteins of the cytochrome p450 from Anopheles gambiae and Αedes aegypti mosquitoes, which associated with insecticide resistance. The technique of HPLC-ESI-MS was used for this analysis, and the response of the system was compared to HPLC-Ortho-SSI-MS. Only one of the three enzymes was not able to metabolise this insecticide. In the last section, Easy Ambient Sonic Spray Mass Spectrometry (EASI or DeSSI-MS) was developed and applied for the rapid analysis of C. reinhardtii cells incubated with sub lethal concentrations of selenium. The main characteristic is that minimal sample preparation is needed for the analysis of cells. Membrane lipids such as phosphatidylglycerol (PG), sulfoquinovosyldiacylglycerol (SQDG) and 1,2- diacylglyceryl-3-O-4’-(N,N,N- trimethyl)-homoserine (DGTS) were detected. The effect of toxic metalloids [Se(IV) και Se(VΙ)] resulted in different profiles of lipids in the mass spectra that possibly indicate membrane lipid double-bond reduction.
Language Greek
Subject High performance Liquid Chromatography
Mass spectrometry
Ιοντισμός υπερηχητικής εκνέφωσης
Υγρή χρωματογραφία υψηλής απόδοση
Issue date 2021-11-24
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/4/e/8/metadata-dlib-1635761554-46174-14791.tkl Bookmark and Share
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