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Identifier |
000334839 |
Title |
Ο ρόλος των σφιγγολιπιδίων, γλυκολιπιδίων και φωσφολιπιδών στο επιθηλιακό φραγμό διαπερατότητας της τραχείας στη Drosophila melanogaster |
Alternative Title |
The role of sphingolipids, glycolipids and phospholipids in the epithelial permeability barrier of the trachea in Drosophila melanogaster |
Author
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Τσικαλά, Γεωργία
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Thesis advisor
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Καραγωγέως, Δόμνα
Δελιδάκης, Χρήστος
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Abstract |
The cellular junctions that connect the neighbouring cells of the epithelia, are important for the maintenance of the epithelial integrity. In polarised cells, junction formation and function depend on
cells attaining the correct apical-basal polarity and on the trafficking of junction components to the correct membrane domain. While much progress has been made in learning how several junction
proteins contribute to cell polarity, little is known about the contribution of lipids to cell polarization and cell adhesion.
We use Drosophila as a system to study the role of lipids ((glyco)-sphingolipids and phospholipids) in the establishment of cell polarity and cell adhesion. We concentrate on the
formation of septate junctions (SJ), which sustain the permeability diffusion barriers, and are found
among epithelial cells and among glial cells that enwrap the nervous system. As a handle to the
genetic study of lipids in this context, we investigate the contribution of lipid metabolism genes to the
process of SJ formation. We performed a genetic screen for the identification of new genes
responsible for integrity of the epithelia permeability barrier in Drosophila melanogaster, using the
dye penetration assay, which is an easy way to monitor the disruption of the epitheial permeability
barrier. We screened mutants in sphingolipid, glycosphingolipid, and phospholipid metabolism genes
and identified 8 possible candidate genes, which when mutated, lead to disruption of the permeability
barrier.
In this particular study, we are trying to phenotypically characterize two mutant strains that show
impaired permeability barrier in the dye penetration assays. Additionally, in the context of studying a
lipid metabolism gene that appears to be involved in the development of the trachea, we are trying to
characterize a mutant strain for this gene.
A second part of this project involves the characterization of the subcellular localization of
different types of lipids in polarized cells of the epithelia of Drosophila melanogaster, by creating
adequate chimeric molecules.These molecules contain GFP protein and allow the speculation of
different types of lipids.
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Language |
Greek |
Subject |
Cell Communication |
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Intercellular Junctions |
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Lipid |
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Lipid sensors |
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Septate junctions |
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Αισθητήρες λιπιδίων |
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Διαφραγματικοί σύνδεσμοι |
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Κυτταρική επικοινωνία |
Issue date |
2008-12-04 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Notes |
Διατμηματικό Μεταπτυχιακό Πρόγραμμα Μοριακής Βιολογίας- Βιοΐατρικής |
Permanent Link |
https://elocus.lib.uoc.gr//dlib/e/e/0/metadata-dlib-3bcf3c376ce24b38b3b127165655fd71_1235023811.tkl
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Views |
376 |