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|
Identifier |
000360019 |
Title |
Εφαρμογή της κυτταρομετρίας ροής για τον ποσοτικό χαρακτηρισμό των κυτταρικών πληθυσμών του αμφιβληστροειδή αρουραίου |
Alternative Title |
Quantification of neuronal cells in healthy rats retinas by flow cytometry |
Author
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Τσόκα, Παυλίνα
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Thesis advisor
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Τσιλιμπάρης, Μιλτιάδης Κ.
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Reviewer
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Θερμού, Κυριακή
Γραβάνης, Αχιλλέας
|
Abstract |
The primary purpose of this study was to evaluate the potential to quantify the
different neuronal populations in the retina of healthy Sprague–Dawley rats in
an accurate quantitative way by using flow cytometry. Rats were killed and the
eyes were enuclated to achieve retinal dissection and then dissociation by
trypsin. The cells were mechanically dissociated into a single-cell suspension.
The dissociated cells were permeabilized and stained with the primary
antibody and afterwards with the secondary. Αt least 100.000 healthy cells
were analyzed with a FACScalibur and FlowJo software. Dead cell and cell
debris were exluded from analysis by gating with forward scatter (FCS, cell
size) and side scatter (SSC, cell complexity) as indicators. The primary
antibodies were anti-rhodopsin against photoreceptors, anti-Protein Kinace C
against bipolar cells, anti-calbindin against horizontal cells and antimicrotubule
associated protein 1 against ganglion cells. Quantification of the
above populations was possible using flow cytometry. In this study, the
photoreceptors had the 53.99%, the ganglion the 7%, the bipolars the 2%, the
horizontal the 1.5% in the hole mixed retinal population. The experiments
were repeated three times and these measurements are the mean value for
each cell category. Flow cytometry can be used to quantify the different
neuronal populations in control healthy eyes and this verification will be very
useful in future in studies in apoptosis of these cells.
|
Physical description |
83 σ : πιν. ; 30 εκ. |
Language |
Greek |
Subject |
Flow optometry |
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Neuronal cells |
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Ophthalmology |
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Retina |
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Αμφιβληστροειδής |
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Κυστικό οίδημα ωχράς |
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Κυτταρικοί πληθυσμοί |
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Κυτταρομετρία ροής |
Issue date |
2009-04-07 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
|
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Type of Work--Post-graduate theses
|
Notes |
Διατμηματικό μεταπτυχιακό πρόγραμμα σπουδών : "Οπτική και Όραση". |
Permanent Link |
https://elocus.lib.uoc.gr//dlib/a/0/b/metadata-dlib-adfa2d298eb348c80ee39ab7530cb72e_1283936386.tkl
|
Views |
263 |