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Identifier	000345752
Title	Συμβολή της γενετικής και βιοτεχνολογίας στην κατανόηση της αποφρακτικής νόσου των αεραγωγών
Author	Ζερβού, Μαρία Ι.
Thesis advisor	Σιαφάκας, Νικόλαος
Reviewer	Γεωργόπουλος, Δημήτριος Φρουδαράκης, Μάριος Ζαννής, Βασίλειος Μπούμπας, Δημήτριος Καρδάσης, Δημήτριος Τζανάκης, Νικόλαος
Abstract	Previous studies have shown that microsatellite (MS) DNA instability (MSI) is detectable in sputum cells in chronic obstructive pulmonary disease (COPD) and asthma. The aim of the present study was to investigate whether asthma and COPD could be distinguished at the MS DNA level. DNA was extracted from sputum cells and white blood cells from 63 COPD patients, 60 non-COPD smokers, 36 asthmatics and 30 healthy non-smokers. Ten MS markers located on chromosomes 2p, 5q, 6p, 10q, 13q, 14q and 17q were analysed. No MSI was detected in non-COPD smokers or healthy nonsmokers. A significantly higher proportion of COPD patients exhibited MSI (49.2%) compared to asthmatics (22.2%). MSI was detected even in the mild stages of COPD (33.3%) and asthma (22.2%). No relationship was found between MSI and COPD severity. The most frequently affected marker was D14S588 (17.5% in COPD and 2.7% in asthma). The markers D6S344, G29802 and D13S71 showed alterations only in COPD, and G29802 was associated with a significantly decreased forced expiratory volume in one second FEV1 (% predicted), whereas MSI in D6S344 was associated with a significantly higher FEV1 (% pred). The frequency of microsatellite instability was higher in chronic obstructive pulmonary disease than in asthma, and microsatellite instability in three workers showed chronic obstructive pulmonary disease specificity. However, further studies are needed to verify the differences between chronic obstructive pulmonary disease and asthma at the microsatellite level.
Physical description	148 σ. : πιν. ; 30 εκ.
Language	Greek
Subject	Pulmonary Disease, Chronic Obstructive
	Respiratory System
Issue date	2007-07-26
Collection	School/Department--School of Medicine--Department of Medicine--Doctoral theses
	Type of Work--Doctoral theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/6/1/1/metadata-dlib-babfbe412a90d124e02ba8970f9e0f62_1243410195.tkl
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