E-Locus - Institutional Repository of the University of Crete

Home Search

Results - Details

Search command : Author="Γανωτάκης" And Author="Δημήτριος"

Current Record: 21 of 100

[Add to Basket]

Identifier

000441521

Title

Development of obligate fermentative Escherichia coli srains

Alternative Title

Ανάπτυξη υποχρεωτικά ζυμωτικών στελεχών Escherichia coli

Author

Ανδρεαδάκη, Θεοφανία Παγώνα Ι.

Thesis advisor

Γανωτάκης, Δημήτριος

Reviewer

Τσιώτης, Γεώργιος
Παυλίδης, Ιωάννης

Abstract

The aim of this work was the development and study of obligate fermentative Escherichia coli strains. An obligate fermentative strain derived from SIJ488 was studied both in the presence of various single carbon sources such as glucose, glycerol, acetate, pyruvate, and in a combination of glucose with pyruvate. This new strain was furtherly evolved, providing interesting sequencing information, and thus contributing to the deeper understanding of its fermentative metabolism with the support of techniques such Reverse Transcriptase-quantitative PCR. Νew strains were generated either to mimic the evolution or to provide deeper understanding of the metabolism of the mutated strains. λ- Red Recombination was used for the gene deletions. The new strains were studied in the presence of various carbon sources such as glucose, glycerol, acetate, or pyruvate. Ion exchange chromatography was used for the quantification of the fermentation products and nuclear magnetic resonance analysis was used for additional product confirmation. Morphological studies by Scanning Electron Microscopy and negative staining of the strains also carried out. Thus, information was acquired on how their engineered metabolism affects the morphology of the cell by causing shape elongation and loss of flagella. Finally, another two obligate fermentative strains were developed with mostly the gene deletion method P1- Phage transduction.

Language

English

Subject

Aerobic conditions

Electron transport chain

Escherichia coli SIJ488

Fermentation

Ion Chromatography

Lactate

NMR

Respiration

Ubiquinone