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Identifier

000426430

Title

Study of transporters in Nezara viridula: insights for pest control and insecticide transport

Alternative Title

Μελέτη μεταφορέων στη Nezara viridula: συνεισφορά στην καταπολέμηση του εντόμου και τη μεταφορά εντομοκτόνων

Author

Δρίβα, Ολυμπία Α.

Thesis advisor

Βόντας. Ιωάννης

Reviewer

Δελιδάκης, Χρήστος
Σαρρής, Παναγιώτης

Abstract

Nezara viridula is an important cosmopolitan polyphagous pest of horticultural and agricultural importance that is currently controlled mainly with the use of insecticides. These small molecules are transported through barrier tissues like the midgut is by transporter proteins belonging in the ABC and SLC superfamilies. Members of these families also have core physiological roles, and have been proposed as drug targets in other species. This study therefore focused on the identification and characterization of small molecule transporters of the ABC and SLC superfamilies in terms of their capacity to act as targets and transporters of insecticides. For the first part of this study we have developed a bioassay that allowed us to calculate the baseline oral toxicity of different insecticides. This bioassay will serve in the future as a tool to assess changes in this baseline toxicity after the manipulation of the expression of proteins that transport insecticides. We have also identified two new putative drug targets belonging in the ABC family: knocking them down with RNAi resulted in lethality or developmental arrest. For the second part of the study we have identified two ABC and one SLC transporters that might have a role in transporting insecticides, and made the first steps for their characterization using RNAi and heterologous expression systems. Finally, the possible case of horizontal gene transfer of a bacterial SLC47 to the N. viridula genome was examined. Overall these studies will help us better understand the insect-insecticide interaction, as well as design new pest control strategies.

Language

English

Subject

ABC transporters

ABC μεταφορείς

Insecticide pharmacokinetics

SLC transporters

SLC μεταφορείς

Φαρμακοκινητικές ιδιότητες εντομοκτόνων

Issue date

2019-11-29

Collection