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Identifier

000433274

Title

Παραγωγή πολυ(3-υδρόξυ βουτυρικού) εστέρα (PHB) από το βακτηριακό στέλεχος Pseudomonas sp. phDV1 παρουσία φαινόλης

Alternative Title

Production of poly(3-hydroxybutyrate) ester (PHB) by the bacterial strain Pseudomonas sp. phDV1 in the presence of phenol

Author

Καναβάκη, Ηλιάνα Μ.

Thesis advisor

Τσιώτης, Γεώργιος

Reviewer

Γανωτάκης. Δημήτριος
Παυλίδης, Ιωάννης

Abstract

The bacterium Pseudomonas sp. phDV1 is a Gram-negative bacterium that has the ability to grow in the presence of phenol and use it as a carbon source by breaking it down through the meta degradation pathway. The aim of the present study was to study this bacterium in terms of its ability to produce poly(3-hydroxybutyrate) (PHB) in the presence of phenol. Initially, a protocol for the isolation of polyhydroxyalkanoates (PHA) from cells was developed and then an attempt was made to optimize the culture conditions of the bacterium by varying the duration of growth as well as the concentration of phenol in the medium. The best conditions from those tested were found to be 72 hours of culture and 600 mg/L concentration of phenol in the medium. After isolating the solid produced, an attempt was made to characterize it by infrared spectroscopy (IR), nuclear magnetic resonance spectroscopy (NMR) and matrix-assisted laser desorption ionization (MALDI) techniques. The presence of characteristic peaks in the IR and NMR spectra as well as the presence of ions with the molecular weight of the monomer in the mass spectrum, are strong indications that the polyhydroxyalkanoate is the poly-(3-hydroxybutyrate). An attempt was then made to identify poly-(R)-hydroxyalkanoic acid synthase with nLC-MS, as well as kinetic study of the enzyme at 24.6 ^oC and 32 ^oC which is the optimal growth temperature of the bacterium.

Language

Greek

Subject

Biodegradation

Biopolymers

Polyhydroxyalkanoates

Βιοαποικοδόμηση

Βιοπολυμερή

Πολυυδροξυαλκανοϊκά

Issue date

2020-11-27

Collection