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Identifier 000403424
Title Συγκριτική απόκριση σε αβιοτικές καταπονήσεις γενετικά τροποποιημένων φυτών Arabidopsis thaliana που υπερ/υποεκφράζουν τα AtPAO1, 3, 5 γονίδια
Alternative Title Comparative response to abiotic stresses of genetically engineered Arabidopsis thaliana plants over/under expressing the AtPAO1, 3, 5 genes
Author Παγουλάτου, Μαριάνθη Γ.
Thesis advisor Κοτζαμπάσης, Κυριάκος
Reviewer Ρουμπελάκη-Αγγελάκη, Καλλιόπη
Χαλεπάκης, Γεώργιος
Abstract The elucidation of the molecular mechanisms that confer resistance to stresses is an cutting’ edge topic in plant biology, because their understanding can lead to the development of tolerant genotypes. The contribution of polyamines (PAs) in growth, development and stress responses has been well documented tduring the recent years. Their mode of action an open question. A key factor seems to be the polyamine oxidases (PAOs) generated hydrogen peroxide ( Η2Ο2). Depending on its cellular titers it can signal either plant defence mechanisms or the programmed cell death syndrome. In the genome of dicotyledonous model-plant Arabidopsis thaliana, five PAO genes (AtPAO1 to AtPAO5) have been characterized encoding proteins with different subcellular localization. The AtPAO2, 3 and 4 are localized in peroxisome while AtPAO1 and AtPAO5 are cytoplasmic. All AtPAOs back-convert PAs, converting Spm (or T-Spm) to Spd (partial back-conversion), or Spm (or T-Spm) first to Spd and then to Put (full backconversion). The purpose of this study was to investigate whether deregulation of AtPAOs gene expression, can affect cell homeostasis under normal, salinity and heavy metal stress conditions. In doing so we used Arabidopsis thaliana plants over-expressing PAO (SAtPAO1, 3, 5), the respective mutants (Atpao1, 3, 5) and wild-type plants (Col-0, WT). Our results support that overexpression of peroxisomal AtPAO3 (S-AtPAO3) confers resistance to stress, more likely by inducing expression of the antioxidant genes resulting in the scavenging of Reactive Oxygen Species and by increasing the levels of PAs, whereas, the under-expression (Atpao3), makes it sensitive. On the contrary, overexpression of the cytoplasmic AtPAO1 (S-AtPAO1) results to sensitive genotype, whilst under-expression (Atpao1) confers resistance. AtPAO3 appears to be indispensable in maintaining cellular homeostasis, since its absence causes deregulation at many levels.
Language Greek
Subject Abiotic Stress
Polyamine Oxidases
Αβιοτική καταπόνηση
Πολυαμινικές οξειδάσες
Issue date 2016-11-18
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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