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Identifier |
000431679 |
Title |
Διερεύνηση υπογραφών έκφρασης miRNA προς διαστρωμάτωση ασθενών με γλοιοβλάστωμα |
Alternative Title |
Deciphering miRNA signatures toward patient stratification in glioblastoma multiforme |
Author
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Μπαφίτη, Παρασκευή
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Thesis advisor
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Κατσίλα, Θεοδώρα
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Reviewer
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Πίντζας, Αλέξανδρος
Τσατσάνης, Χρήστος
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Abstract |
Malignant gliomas constitute a complex disease phenotype that demands optimum decision-making. Despite being the most common type of primary brain tumors, gliomas are highly heterogeneous when their pathophysiology and response to treatment are considered. Such inter-individual variability also renders optimum patient stratification extremely difficult.
Recent evidence highlight that the gene-environment interplay becomes of fundamental importance in oncogenesis and progression of gliomas. For glioblastoma multiforme, in particular, genotype-to-phenotype associations are critical.
Herein, we put emphasis on a microRNA signature (hsa-miR-20a, hsa-miR-21, hsa-miR-10a) to map the inter-individual variability of glioblastoma multiforme patients toward optimum patient stratification.
Data and text mining reported a key role for hsa-miR-20a, hsa-miR-21 and hsa-miR-10a in the regulation of gene expression in glioblastoma pathophysiology. Next, immunohistochemistry analysis of FFPE tissue samples of glioblastoma multiforme patients (n=37) was performed for TGFb-RII, hMSH2 and EphA8. microRNA expression levels were determined by RT-qPCR.
hsa-miR-20a, hsa-miR-21 and hsa-miR-10a were up-regulated. Such findings are further supported by our immunohistochemistry analysis for TGFb-RII, hMSH2 and EphA8. Molecular-clinical correlations were drawn to classify high- vs. low-risk patients, shedding light to the molecular mechanisms involved in disease progression. This microRNA signature (hsa-miR-20a, hsa-miR-21, hsa-miR-10a) may further empower evidence-informed decision-making in clinical practice.
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Language |
Greek, English |
Subject |
Metabotype |
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Μεταβότυπος |
Issue date |
2020-08-05 |
Collection
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School/Department--School of Medicine--Department of Medicine--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/c/6/3/metadata-dlib-1598960794-365343-29358.tkl
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Views |
290 |