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Identifier 000401793
Title Μελέτη μηχανισμών αποτοξικοποίησης εχθρών καλλιεργειών, μέσω RNAi σίγησης σε διαγονιδιακά φυτά και λειτουργικής έκφρασης ανασυνδυασμένων ενζύμων.
Alternative Title Investigation of resistance mechanisms of agricultural pests
Author Πιτσιλή Ευγενία Κ.
Thesis advisor Βόντας, Ιωάννης
Reviewer Καλαντίδης, Κρίτων
Τσαγρή Ευθυμία
Abstract Spider mite Tetranychus urticae and whitefly Bemisia tacabi are two of the most damaging pests in agriculture. Chemicals are the main way to control their populations. However, their extensive use is responsible for the selection of extremely high levels of insecticide/ acaricide resistance for both species. Aim of this study is to elucidate the resistance mechanisms as it is an important pre-requirement for the management of resistance. The study is composed by two chapters. In the first chapter of the thesis we tried to determine the detoxification mechanisms of T. urticae which are caused by the effect of P450s, through transgenic plants Solanum lycopersicum. These plants express double- stranded small RNA and target the cpr gene (reductase of cytochrome P450s proteins and necessary for their function). RNAi technology has been employed recently to develop plants with improved resistance to agricultural pests. The transgenic plants were checked for the expression of small dsRNAs of the CPR gene which were delivered to the mites through feeding from the plants and finally we analyzed the expression levels of CPR gene with real time PCR. No efficient silencing effect was observed. Populations of the whitefly Bemisia tacabi collected from greenhouses in Crete exhibited high levels of resistance against different neonicotinoid insecticides. Previous studies showed correlation between insecticide resistance and over- expression of P450s. In the second chapter we examined the capability of P450s to metabolize two important neonicotinoids, Imidacloprid and Acetamiprid. Two genes, cyp6cx3 and cyp6cx5 were cloned to the appropriate vectors for protein expression and recombinant proteins were expressed. Under the specific conditions that the experiment was conducted, no metabolism of insecticide was detected.
Language Greek
Subject Acari
Bemisia tacabi
Insecticides - Pesticides
P450s
Ακαρεοκτόνα - Εντομοκτόνα
Αλευρώδης κυτοχρωμικές οξειδάσες
Τετράνυχος
Issue date 2016-07-22
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/8/1/9/metadata-dlib-1467632708-730707-30349.tkl Bookmark and Share
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