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Identifier 000364214
Title Δείκτες έκθεσεις σε φυτοφάρμακα μελέτη εναπόθεσης οργανοχλωριωμένων και οργανοφωσφορικών φυτοφαρμάκων σε τρίχες πειραματόζωων και επέκταση αυτής στον άνθρωπο
Alternative Title Biomarkers of exposure to pesticides,study of incorporation of organochlorine and organophoshate pesticides in hair from experimental animals and extrapolation of the study to humans.
Author Μαργαρίτη, Μαρία
Thesis advisor Τσατσάκης, Αριστείδης
Reviewer Χανιωτάκης, Νίκος
Τζανακάκης, Γιώργος
Κογεβίνας, Εμμανουήλ
Σπαντίδος, Δημήτριος
Τσακάλωφ, Ανδρέας
Ζαφειρόπουλος, Αλέξανδρος
Abstract Αn analytical approach for the quantitative determination of three dialkyl phosphate (DAP) metabolites, dimethyl phosphate (DMP), dimethyl thiophosphate (DMTP) and diethyl phosphate (DEP), of organophosphate pesticides (OPs) in hair samples has been developed. The proposed methodology comprises a decontamination step, solid-liquid extraction, followed by liquid-liquid extraction, pentafluorobenzyl bromide derivatization, clean-up on Florisil/PSA column and analysis by gas chromatography-mass spectrometry (GC-MS). The analytes were extracted from hair with water. Two-coloured rabbits were daily exposed to dimethoate (0, 12 or 24 mg kg-1 body weight) via their drinking water and their hair samples, obtained 4 and 6 months after the beginning of exposure, were analyzed for the presence of its two metabolites (DMP, DMTP). The influence of dose and dose duration of this OP, as well as the effect of hair colour on the concentrations of its DAPs in hair were investigated. To test the efficiency of the method, a 30 samples of hair from agricultural workers were analyzed. The procedure was shown to be linear over a wide range of concentration. Satisfactory recoveries were obtained ranging from 58.8 to 101.4% for rabbit hair and from 56.1 to 107.9% for human hair, whereas the limits of detection ranged from 0.01 to 0.08 ng mg-1 and from 0.02 to 0.10 ng mg-1 for rabbit and human hair, respectively. The present work shows that there appears to be a significant relationship between the dose of dimethoate and the concentration values of its two DAP metabolites in hair. On the other hand, mixed results for the significance of the relationship between dose duration and the levels of the two DAPs in hair are observed. Hair pigmentation does not appear to affect the concentration values of DMTP, whereas it seems to be a critical factor in the incorporation of DMP into hair. The results obtained from the analysis of hair samples of agricultural workers show the suitability of the proposed method for monitoring people occupationally exposed to OPs. DEP was the most frequently detected metabolite in hair samples from agricultural workers, followed by DMP. These data confirm the ability of hair testing to assess chronic OP exposure by the detection of DAPs.
Language Greek
Subject Hair
Pharmacology
Poisons
Δηλητήρια
Τριχες
Φαρμακολογία
Issue date 2009-07-24
Collection   Faculty/Department--School of Medicine--Department of Medicine--Doctoral theses
  Type of Work--Doctoral theses
Permanent Link https://elocus.lib.uoc.gr//dlib/6/1/7/metadata-dlib-87ce8ef05e07183799094e0f53ef541f_1298362701.tkl Bookmark and Share
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