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| Title |
Προσδιορισμός των επιπέδων σουλφοναμίδων και αμινογλυκοσίδων σε δείγματα νωπού κρέατος |
| Alternative Title |
Determination of sulfonamides and aminoglycosides levels in raw meat samples |
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Author
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Αυγενάκη, Μαρία
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Thesis advisor
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Κουτσολέλος, Αθανάσιος
Τσατσάκης, Αριστείδης
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| Abstract |
Antibiotics are an integral part of systemic farming of productive animals, while they are
administered in order to control infections, reproductive process and parasites. However,
irrational use of antibiotics results in maintaining residues of these substances in animals’ tissues
while transferring them in dairy products (milk, eggs, meat).
Aiming to the investigation of this phenomenon, in the current study, 54 samples of raw
meat were examined in order to determine the concentration levels of sulfonamides
(sulfamethoxazole, sulfadiazine) and aminoglycosides which are two of the main antibiotics
categories. Beef, pork and chicken were the types of meat that were examined while the tissues
were the muscles, the liver and the kidneys. The analytical methods that were considered most
appropriate were the ELISA and liquid chromatography – mass spectrometry (LC – MS).
The results obtained by ELISA technique showed that all meat samples were positive for
sulfonamides with high percentages of positivity (>80%). On the contrary, streptomycin had not
been detected in all of the samples (only in pork and chicken kidneys and beef muscles and liver)
and the corresponding positivity percentages were much lower (17 – 50 %). Nevertheless, the
mean concentration was higher ranging from 53.44 to 188.88 μg/kg, while for sulfonamides the
mean concentration ranged from 4.82 to 47.22 μg/kg.
LC – MS method was used for the identification of sulfamethoxazole and sulfadiazine. It
was observed that no sample was positive for sulfadiazine while all samples were positive for
sulfamethoxazole except for beef liver and chicken kidney. Positivity percentages for
sulfamethoxazole was 22 – 89 % and the mean concentration levels were 4.49 – 22.42 μg/kg
(range 33.96 – 66.95 μg/kg).
Comparing our results with these from previous studies showed that there was no
accordance concerning beef meat samples as our positivity percentages for streptomycin were
higher. On the contrary, our results for pork samples were in agreement with those of previous
studies presenting similar concentration levels except for pork muscles for which the
sulfonamides levels in our study were much higher.
The current survey highlighted the presence of antibiotics in raw meat. It is of high
importance to study the possibility of these substances to result through the food chain in
consumers’ food stuff.
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| Language |
Greek |
| Issue date |
2020-03-27 |
| Collection
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School/Department--School of Sciences and Engineering--Department of Chemistry--Graduate theses
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Type of Work--Graduate theses
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| Permanent Link |
https://elocus.lib.uoc.gr//dlib/2/1/2/metadata-dlib-1585645230-209850-17760.tkl
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