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Identifier 000423742
Title The role of Thrombospondin 1 in Antiphospholipid Syndrome
Alternative Title Ο ρόλος της θρομβοσπονδίνης 1 στο αντιφωσφολιπιδικό σύνδρομο
Author Τσίκη, Ειρήνη
Thesis advisor Τσατσάνης, Χρήστος
Reviewer Βλαχογιαννόπουλος, Παναγιώτης
Βεργίνης, Παναγιώτης
Abstract Antiphospholipid syndrome (APS) is an acquired thrombophilia characterized by recurrent thrombosis and/or pregnancy morbidity, in the presence of antibodies to β2 glycoprotein-I (β2GPI) or prothrombin. Anti-β2GPI antibodies recognize complexes of β2GPI dimers with CXCL4 chemokine and activate platelets. Thrombospondin 1 (TSP-1) is secreted by platelets and exhibits prothrombotic and proinflammatory properties. Therefore, in this study we investigated the role of TSP-1 in APS. Human Umbilical Vein Endothelial Cells (HUVECs) treated with plasma, total IgG or anti-β2GPI from APS patients and healthy donors were found to secrete TSP-1 as quantified by ELISA and Real-Time PCR. Immunofluorescent stainings for markers of endothelial cell dysfunction and APS were conducted in HUVECs and in placenta from APS patients and Healthy donors. Titration experiments with various concentrations of TSP-1 in HUVECs were also conducted in order to confirm the effect of TSP-1 in the cells. In a parallel clinical and laboratory study, higher plasma levels of TSP-1 were observed in APS but not HDs. Patients with arterial thrombotic events or those undergoing a clinical event had the highest TSP-1 levels. These findings support a possible implication of TSP-1 in APS pathophysiology. In vitro cell treatments along with patient plasma measurements in APS patients suggest that high TSP-1 levels could mark a prothrombotic state and an underlying inflammatory process.
Language English
Subject APS
Issue date 2019-07-17
Collection   Faculty/Department--School of Medicine--Department of Medicine--Post-graduate theses
  Type of Work--Post-graduate theses
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