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Identifier |
000460268 |
Title |
Functional cell-based assays for the characterization of putative insecticide resistance mutations in the β-adrenergic like octopamine receptor (Octβ2R) in arthropod pests |
Alternative Title |
Λειτουργικές κυτταρικές δοκιμασίες για τον χαρακτηρισμό πιθανών μεταλλαγών ανθεκτικότητας σε εντομοκτόνα στον β-αδρενεργικό υποδοχέα οκτοπαμίνης (Octβ2R) σε αρθρόποδα παράσιτα |
Author
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Κατσάνου, Αικατερίνη Α.
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Thesis advisor
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Βόντας, Ιωάννης
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Reviewer
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Δουρής, Βασίλειος
Γκουρίδης, Γεώργιος
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Abstract |
Dermanyssus gallinae, also known as the Poultry Red Mite (PRM), is a
destructive ectoparasite found in poultry farms, with a significant presence in Europe
and other regions. These mites feed primarily on broiler birds, leading to economic
losses in the egg production industry. PRM infestation has direct and indirect effects
on poultry and human health. Direct effects include stress, decreased egg production,
and quality, while indirect effects result from the transmission of viruses and bacteria.
This poses a significant challenge for poultry welfare and food safety.
Various methods have been investigated to control PRM populations. Among
them are biological methods, physical interventions, vaccination approaches,
chemical control and others have been studied. However, chemical approaches, while
effective, face challenges such as resistance and food safety concerns. Amitraz, widely
employed against various pests, has encountered resistance issues due to its extensive
use. Resistance to amitraz is associated with genetic mutations in octopamine
receptor genes, affecting the efficacy of the acaricide. This resistance has been
observed in mite species like Tetranychus urticae and Dermanyssus gallinae.
Understanding the genetic and molecular basis of resistance is crucial for developing
effective control strategies.
This thesis aims to validate the impact of β-adrenergic-like octopamine
receptor A89V mutation identified in amitraz-resistant PRM populations (Katsavou E
unpublished data). Our methodology involved the cloning of OAR genes, transfection
optimization, establishment of stable cell lines, and activity assays to evaluate the
impact of the A89V mutation on the activation of Octβ2R upon binding of amitraz and
DPMF. Concluding, statistical analysis revealed that there are significant differences
between the activity levels of the wild-type receptor and the one carrying the A89V
mutation, in the presence of either amitraz or DPMF. Notably, we developed a
heterologous protein system in HEK293 cells, that can be utilized for the investigation
of various resistance mechanisms.
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Language |
English |
Subject |
Activity assays |
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Mammalian cells |
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Parasites |
Issue date |
2023-11-24 |
Collection
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School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
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Type of Work--Post-graduate theses
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Permanent Link |
https://elocus.lib.uoc.gr//dlib/0/c/2/metadata-dlib-1699359338-904260-26923.tkl
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Views |
1226 |
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