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Identifier	000452285
Title	Αξιολόγηση αντιγονικών πρωτεϊνών και μεταβολομική μελέτη του παθογόνου Legionella pneumophila
Alternative Title	Evaluation of antigenic proteins and metabolomic analysis of the pathogen Legionella pneumophila
Author	Πυλούδη Αναστασία-Μαρίνα Δ.
Thesis advisor	Τσιώτης, Γεώργιος
Reviewer	Ψαρουλάκη, Άννα Σπύρος, Απόστολος
Abstract	Legionella is a genus of pathogenic Gram-negative, obligate aerobic bacteria. The species L. pneumophila causes Legionnaires' disease or legionellosis, which presents clinically in the form of two syndromes: an acute bacterial respiratory infection, manifested mainly by pneumonia, and Pontiac fever, a respiratory virus similar to influenza. In the work, proteins of the bacterium L.pneumophila were isolated after heterologous expression in E. Coli. The proteins were evaluated for their antigenicity with sera from patients and healthy individuals. Based on antigenicity, dihydrolipoamide succinyltransferase (lpg_0533) was chosen for the creation of a diagnostic test, which works on the basis of electrochemical impedance spectroscopy. In addition, metabolomic analysis by nuclear magnetic resonance spectroscopy was performed on bacterial cell extract from cultures of the bacterium, to study the metabolites in the different stages of the pathogen's development.
Language	Greek, English
Issue date	2022-11-24
Collection	School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
	Type of Work--Post-graduate theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/b/a/9/metadata-dlib-1669106280-784567-856.tkl
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