E-Locus - Institutional Repository of the University of Crete

Home Search

Results - Details

Search command : Author="Κατερινόπουλος" And Author="Χαράλαμπος"

Current Record: 33 of 79

[Add to Basket]

Identifier

000412962

Title

Απομόνωση και χαρακτηρισμός συστατικών του είδους Hypericum empetrifolium

Alternative Title

Isolation and indentification of ingredients from the species Hypericum empetrifolium

Author

Χριστοφόρου, Γεωργία Μ.

Thesis advisor

Κατερινόπουλος, Χαράλαμπος

Reviewer

Περγαντής, Σπύρος
Σπύρος, Απόστολος

Abstract

The species Hypericum empetrifolium (Clusiaceae) is a herb that grows in Greece and is abundant at the mountainous areas of Crete. Its use in the traditional medicine by the local population as a therapeutic agent against the disease of psoriasis prompted our research group to investigate its ingredients and identify the active components. The isolation procedure was as follows. The plant was collected during the blooming period and dried. The samples were pulverized and the components were extracted in a Soxhlet apparatus with the sequential use of three solvents: hexane, for the extraction of the non-polar ingredients, dichloromethane for the medium polarity components, and methanol for the polar ingredients. Furthermore, the essential oil was collected via hydrodistillation, using a Clevenger apparatus. The three extracts were subjected to fractionation by further extractions and flash chromatography. Analysis of the components was performed using nuclear magnetic resonance spectroscopy (NMR), liquid chromatography coupled with mass spectrometry (LC/MS) gas chromatography coupled with mass spectrometry (GC/MS) and high resolution mass spectrometry (HRMS). The substances identified in the essential oil using GC/MS, amounted to 90.5% of the total components. The major components were: α-pinene (24.52%), allo- aromadenrine (12.08%), β-pinene (6.59%), limonene (6.15%), β-caryophyllene (5.87%) and β-farnesene (4.30%). In addition, the less polar components, obtained via fractionation of the first three extracts, were identified via GC/MS chromatography. Finally, five substances were isolated from the polar extracts: quercetin, chlorogenic acid methyl ester, 3"-acetyl avicularin, quercetin-3-O-galactoside, and neochlorogenic acid methyl ester. 3"-Acetyl avicularin and neochlorogenic acid methyl ester were isolated for the first time from plants of the species empetrifolium and generally the genus Hypericum.

Language

Greek

Subject

Bioactive compounds

Component analysis,

Βιοδραστικές ουσίες.

Issue date

2017-11-23

Collection

School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses

Type of Work--Post-graduate theses

Permanent Link