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Identifier 000388582
Title Ανάπτυξη μεθόδων καταπολέμησης εχθρών καλλιεργειών μέσω σίγησης μηχανισμών αποτοξικοποίησης με διαγονιδιακά φυτά
Alternative Title Plant mediated RNA interference to block detoxification mechanics of agricultural pests
Author Σανδρή, Αναστασία Κ.
Thesis advisor Βόντας, Ιωάννης
Reviewer Καλαντίδης, Κρίτων
Τσαγρή, Ευθυμία
Abstract The mechanism of RNA silencing is a fundamental mechanism for regulating the expression of genes conserved in eukaryotes. In recent years, RNA silencing is used to control agricultural pests. Two of the most damaging pests in agriculture are whitefly Bemisia tabaci and spider mite Tetranychus urticae. Aim of this project is to determine the detoxification mechanisms of these two pests, which are caused by the effect of P450s, through transgenic plants Nicotiana tabacum and Solanum lycopersicum. These plants express double – stranded RNA for the CPR gene. CPR is the reductase of cytochrome P450 proteins which are responsible for resistance to insecticides and plant allelochemicals. In the first chapter, the inserts of CPR gene in transgenic tomato plants were confirmed. Moreover, the constructions of plasmids and transgenic tomato plants for GFP as control gene are described. In the second chapter, the transgenic plants were checked for the expression of small dsRNAs of the CPR gene. The next steps of this project are the phenotypic determinations of pests at the transgenic plants, the analysis of the expression levels of CPR gene with real time PCR and bioassays with insecticides in order to determine the effect of cytochrome P450 suppression in sustainability and resistance of these two harmful pests.
Language Greek
Subject Bemisia tabaci
Tetranychus urticae
Issue date 2014-11-21
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
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