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Identifier

000377737

Title

Η ανίχνευση του ιού του ανθρώπινου θηλώματος (HPV) σε ασθενείς με καρκίνο ουροδόχου κύστεως με τη μέθοδο της ποσοτικής αλυσιδωτής αντίδρασης με πολυμεράαση (real-time quantitative PCR)

Alternative Title

The detection of human papilloma virus (HPV) in patients with urinary bladder cancer using a quantitative polymerase chain reaction (real-time quantitative PCR) technique

Author

Παναγιωτάκης, Γεώργιος Ι

Thesis advisor

Σπαντίδος, Δημήτριος Α

Reviewer

Σουρβίνος, Γεώργιος
Τσατσάνης, Χρήστος
Ζώρας, Οδυσσέας
Τσατσάκης, Αριστείδης
Ζαφειρόπουλος, Α.
Μαμουλάκης, Χ.

Abstract

The aim of the present PhD thesis was to estimate the detection of human papillomavirus (HPV) in tumourigenesis of human urinary bladder, using the Real Time quantitative polymerase chain reaction (Real-Time qPCR) and the effectiveness of this method, in order to be a future safe protocol for the detection of HPV in patients with bladder cancer. Furthermore we studied the detection of human herpesviruses and Polyoma viruses (BK and JC) in the same patients, using the method of polymerase chain reaction. For this PhD thesis we used 30 specimens of tumour tissue and 30 specimens of adjacent healthy bladder tissue, which originated from patients who underwent surgical transurethral resection at the Department of Urology, Asklipeiion General Hospital, Voula, Athens, Greece. We failed to detect positive samples for HPV infection, not only from cancerous bladder specimens, but also from adjacent healthy bladder tissue, although we used three different methods for detection of genetic material of the virus, with progressively increasing sensitivity (PCR, nested-PCR, RTqPCR) We obtained different results trying to detect human herpesvirus and Polyoma virus genome, using the polymerase chain reaction (PCR) technique. For HSV-1, HSV-2, VZV and HHV-7 both tumour and normal specimens, were negative for the detection of the presence of viral specific genome. Cytomegalovirus DNA was detected in 22 tumour samples and 23 normal samples. 17 paired samples were also detected for CMV DNA. EBV DNA was detected in 15 tumour samples and 4 normal samples. 1 paired sample was also detected for EBV DNA. HHV-6 DNA was detected in 11 tumour samples and 1 normal sample. 3 paired samples were also detected for HHV-6 DNA. KSHV DNA was detected in 1 tumour sample and 2 normal samples. None paired sample was detected for KSHV DNA. Polyoma BK and JC viral DNA was detected in 4 tumour samples. None normal sample was positive for the detection of Polyoma BK and JC viral DNA. None paired sample was detected for Polyoma BK and JC viral DNA. Meanwhile EBV DNA presence was abundant in tumour samples compared to normal tissue with statistical significance (p=0,0048). A small yet notable percentage of samples examined (10%) contained CMV and HHV-6 (6,7%), CMV and EBV (3,3%) sequences raising the possibility of synergism between these viruses in urinary bladder carcinogenesis.

Language

Greek

Subject

HPV

Real time quantitative PCR

Urinary Bladder Neoplasms

Καρκίνος ουροδόχου κύστεως

Issue date

2012-12-11

Collection

School/Department--School of Medicine--Department of Medicine--Doctoral theses

Type of Work--Doctoral theses