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Identifier 000381422
Title Μελέτη της βιοαποικοδόμησης αρωματικών ενώσεων από το μικροφύκος Chlamydomonas reinhardtii
Alternative Title Biodegradation study of aromatic compounds from the microalgae Chlamydomonas reinhardtii.
Author Νάζος, Θεοχάρης Θ
Thesis advisor Γανωτάκης, Δημήτριος
Reviewer Κοτζαμπάσης, Κυριάκος
Τσιώτης, Γεώργιος
Abstract In this project, the ability of the microalgae Chlamydomonas reinhardtii to biodegrade some phenolic compounds, which belong to the family of aromatic compounds, was studied. It was found that the wild type strain (CC-125) is able to fully biodegrade the simplest phenolic compound, phenol, in the absence of an alternative carbon source. Phenol had no significant effect on the photosynthetic apparatus of Chlamydomonas. The biodegradation of phenol is not a gradual process but it occurs relatively fast after one day. Immobilized cells of CC-125 in alginate beads, exhibited a very low biodegradation ability of phenol. The ability of the mutant strain S264K to biodegrade phenol was also studied and it was found that, although its photosynthetic apparatus remains intact during the experimental period, the biodegradation efficiency was much lower compared to the CC-125 strain. The biodegradation of phenolic compounds containing an additional group on the aromatic ring was much more difficult for Chlamydomonas reinhardtii. In this case the largest biodegradation percentage is achieved mainly by co-metabolism. Mono-substituted chlorophenols and a series of para-halogenated phenols were used as substrates. In addition, the effect of the nature of the para-substituent (electron donor or acceptor) on the photosynthetic properties of the cells was also studied The main conclusion of this study is that Chlamydomonas reinhardtii is capable of biodegrading all of the abovementioned compounds, but under various growth conditions depending on the compound used. It was found that the size of the aromatic ring substituent affects the substance biodegradation ability, while its place does not play any role. In addition, the presence of a strong electron donor group on the phenolic ring (e.g. nitro-group) renders the compound toxic for the microalgal cells and thus the biodegradation ability decreases. For almost all the phenolic compounds used in this study, it was shown that the biodegradation of each compound requires a specific set of growth conditions in order to be achieved. Apparently the microalgae, depending on 12 the growth conditions, follow the most "profitable" strategy. When it has to choose between growth and biodegradation, and it always chooses the process that is energetically more favorable for the cells. This study contributes some new results in the field of "green biotechnology"and demonstrates once again that the most important factor in biodegradation is the selection of the appropriate conditions.
Language Greek
Subject Compounds
Induced fluorescence
para-substituted phenolic
Επαγωγικός φθορισμός
Φαινολικές ενώσεις
Issue date 2013-11-15
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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