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Identifier 000438445
Title The effect of physicochemical properties of polycaprolactone (PCL) scaffolds on the adhesion, proliferation and differentiation of mouse Mesenchymal Stem Cells (MSCs)
Alternative Title Η επίδραση των φυσικοχημικών ιδιοτήτων ικριωμάτων πολυκαπρολακτόνης στην προσκόλληση, τον πολλαπλασιασμό και τη διαφοροποίηση μεσεγχυματικών βλαστικών κυττάρων ποντικού
Author Τσούτσα, Μαρία Ελένη Γ.
Thesis advisor Κουτσολέλος, Αθανάσιος
Reviewer Ρανέλλα, Ανθή
Στρατάκης, Εμμανουήλ
Abstract The increasing demand for biocompatible bone substitutes has made it a priority to tissue engineering and regenerative medicine scientists. Last decades, there is an interest for topographic features on the implants, which have been proven to mechanically regulate cell behavior and functions. The soft lithography techniques provide the opportunity to replicate accurately cell culture patterned surfaces on polymers of interest, where the effect of various topographical cues on cellular functions can be studied. The most studied synthetic polymers in bone tissue regeneration are aliphatic polyesters, due to their advantage of being easily tailored according to the demands. However, there are still some concerns about osteoconductivity, absorption timing and local pH alterations to polymers’ surfaces. In this study, the material of interest is the polycaprolactone (PCL), a biodegradable synthetic polymer that has been widely used to produce 3D scaffolds due to its biocompatibility, biodegradability, structural stability and excellent mechanical properties. Cell culture studies were carried out using mouse Mesenchymal Stem Cells (mMSCs), an important cell source in tissue engineering due to their ability to self-renew, proliferate, and differentiate into a wide range of tissue-specific lineages, including chondrogenic, adipogenic and osteogenic lineages. The aim of this study is to investigate the effect of patterned and non-patterned (flat) PCL surfaces on mMSCs morphology, adhesion, proliferation and osteogenic differentiation. In an attempt to enhance the cell attachment properties of PCL, we have used ultrafast laser patterned surfaces. Ultrafast pulsed laser irradiation is considered as a simple, precise and effective microfabrication method to produce structures of controlled geometry and pattern regularity. In our study, two types of PCL patterned surfaces (low and high roughness) and a PCL nonpatterned (flat) surface were fabricated via soft lithography method, by using such patterned masters. Their topographical features and surface wettability were assessed by Scanning Electron Microscopy (SEM) and static contact angle measurements. In order to study the effect of surface properties on cell behavior, the MSCs were cultured on PCL patterned and non-patterned substrates. Specifically, the effect of topography on cytoskeleton organization (cell shape), the focal adhesion activity and the cell mechanotransduction were studied. Furthermore, the ability of MSCs cultured on patterned PCL surfaces with various stiffness and topographies to differentiate toward osteogenic lineage and to produce a mineralized matrix were evaluated. It is assumed that our tailor-made PCL micro-environments give the opportunity to affect the cellular behavior and seem to be promising in the field of bone tissue engineering and regenerative medicine in the future.
Language English
Subject Cell adhesion
Mechanotransduction
Osteogenic differentiation
Soft lithography
Tissue engineering
Topography
Yes-associated protein (YAP)
mMSCs
Μαλακή λιθογραφία
Μεσεγχυματικά βλαστικά κύτταρα ποντικού (mMSCs)
Μηχανική ιστών
Μηχανική μεταγωγή
Οστεοκύτταρα
Τοπογραφία
Issue date 2021-03-26
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/f/a/b/metadata-dlib-1616069821-225229-28447.tkl Bookmark and Share
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