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Identifier	000412519
Title	Λειτουργική ανάλυση της συνεισφοράς της ανθεκτικότητας δύο μεταλλαγών του τασεοευαίσθητου καναλιού νατρίου ενάντια στα εντομοκτόνα indoxacarb και metaflumizone με τη χρήση της τεχνική CRISPR-Cas9 στη Drosophila melanogaster
Alternative Title	Functional validation of the contribution of two sodium channel target site mutations in sodium channel blocker insecticides resistance by CRISPR-Cas9 genome modification in Drosophila melanogaster
Author	Σαμαντσίδης, Γεώργιος-Ραφαήλ Κ.
Thesis advisor	Βόντας, Ιωάννης
Reviewer	Δελιδάκης, Χρήστος Δούρης, Βασίλης
Abstract	Insecticide resistance emergence have rendered laborious the counteract of agricultural pests and vector borne diseases the last decades. Plutella xylostella and Tuta absoluta comprise two major agricultural pests having a tremendous potential to develop resistance against insecticides such as Sodium Channel Blocker Insecticides (SCBIs, Indoxacarb and Metaflumizone). Two amino acid alterations, F1845Y and V1848I (P.xylostella numbering), in the sixth segment of the forth domain of sodium channel have been correlated with high levels of indoxacarb and metaflumizone resistance. Although electrophysiological studies have shown the correlation of those mutations with resistance against SCBIs, no in vivo experimental data have been recorded so far. In this study we have managed to introduce F1845Y and V1848I mutations in Drosophila melanogaster sodium channel gene (paralytic), with the CRISPR-Cas9 genome editing technique, in order to investigate functionally the contribution to SCBIs resistance. F1845Y and V1848I mutations seem to confer higher resistance to metaflumizone (3460X and 9.3X respectively) than to indoxacarb (12.5X and 6.6X respectively). Moreover we had the question why F1845Y and V1848I mutations have never been found in the same allele in field populations of P.xylostella and T.absoluta. For this reason we tried to introduce both mutations in cis, in order to investigate whether the phenotype is lethal or not. These results provided useful information about the contribution of those mutations to indoxacarb and metaflumizone resistance and suggest that both indoxacarb and metaflumizone exhibit stronger binding affinity to the 1845Y than to 1848I.
Language	English
Subject	Ανθεκτικότητα
	Δροσόφιλα
Issue date	2017-11-22
Collection	School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
	Type of Work--Post-graduate theses
Permanent Link	https://elocus.lib.uoc.gr//dlib/6/e/1/metadata-dlib-1511261550-178569-17437.tkl
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