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Identifier 000454372
Title Development of CRISPR/Cas9-based technologies for genome editing in pancrustacan model organisms
Alternative Title Ανάπτυξη τεχνολογιών CRISPR/Cas9 για τη γονιδιωματική τροποποίηση πανκαρκινοειδών μοντέλων οργανισμών
Author Αρχοντίδης, Θεμιστοκλής Χ.
Thesis advisor Παυλόπουλος, Αναστάσιος
Reviewer Βόντας, Ιωάννης
Δελιδάκης, Χρήστος
Abstract The Clustered Regularly Interspaced Short Palindromic Repeats (CRIPSR) / CRISPR-associated protein 9 (Cas9) genome editing system is a breakthrough technology that provides a simple and accurate way for performing functional genetic analyses in several fields of biological research. This is especially important for studies involving emerging model organisms with limited experimental tools that would benefit greatly from gene knock in and knock out approaches. This project was focused on the development and optimization of different CRISPR/Cas9 knock in strategies on two emerging pancrustacean model species: the coleopteran insect Tribolium castaneum and the amphipod crustacean Parhyale hawaiensis. In the case of Tribolium castaneum, CRISPR knock in with single stranded oligos (ssODNs) was used in order to introduce specific non synonymous mutations in two genes, dld and Cyt-b5-fad, which have been implicated in insect resistance to phosphine. In the case of Parhyale hawaiensis, a CRISPR knock in strategy based on the non-homologous end joining repair mechanismwas selected to test in vivo the UAS/Gal4 binary system for gene expression. Quantitative and qualitative parameters for both approaches are presented and discussed for future experimentation in these pancrustacean models.
Language English
Subject Binary expression system
CRISPR/Cas9 gene editing
Parhyale hawaiensis
Phosphine
Tribolium casteneum
Δυαδικά συστήματα έκφρασης
Πανκαρκινοειδή
Σύστημα γονιδιωματικής τροποποίησης CRISPR/Cas9
Φωσφίνη
Issue date 2023-03-24
Collection   School/Department--School of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/c/3/0/metadata-dlib-1678980808-531218-20443.tkl Bookmark and Share
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