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Identifier

000377911

Title

Η ανίχνευση της έκφρασης των γονιδίων RKIP και RAF (A,B και C) σε ασθενείς με καρκίνο ουροδόχου κύστης

Alternative Title

Implication of RAF and RKIP genes in urinary bladder cancer

Author

Χατζηιωάννου, Μαρία Ν

Thesis advisor

Σπαντίδος, Δημήτριος

Reviewer

Σοφράς, Φραγκίσκος
Τσατσάνης, Χρήστος
Σαμώνης, Γεώργιος
Ζώρας, Οδυσσέας
Σουρβίνος, Γεώργιος
Ζαφειρόπουλος, Α.

Abstract

RKIP has been shown to regulate the RAS-RAF-MEK-ERK kinase cascade acting as modulator of apoptosis and metastasis in prostate cancer. Our goal was to examine the expression of the RAF (A-RAF, B-RAF and RAF-1) and RKIP genes in urinary bladder cancer. Microarray analysis and qPCR was employed to investigate the expression of RAF and RKIP, in 30 patients with transitional cell carcinoma (TCC) of the urinary bladder vs. the corresponding levels of adjacent normal tissue. Computational analysis was also performed on Gene Expression Omnibus (GEO) datasets, to unravel differences in the expression of RAF or RKIP between tumor and control samples, and between superficial and muscle invasive tumors. Microarray analysis revealed gt;2-fold expression of BRAF and RKIP in T2, T3, grade III tumors vs. controls. B-RAF over-expression was verified by qPCR in pT1, grade III tumors vs. their normal counterparts (p=0.016). qPCR revealed a significant RKIP reduction in TCC vs. normal tissue (p=0.002 and plt;0.001 for T1, grade II and Ta-T1, grade III, respectively); All RAF genes were positively correlated among each other (A-RAF/B-RAF, p=0.003; A-RAF/RAF-1, plt;0.001; B-RAF/RAF-1, p=0.050), whereas B-RAF was negatively correlated with RKIP in TCC (p=0.050). Further computational analysis revealed different expression profiles for the genes of interest, among muscle invasive carcinomas, superficial TCCs, cystectomy specimens and normal tissue. The reduced RKIP mRNA levels in TCC and the elevated levels of B-RAF in pT1, grade III tumors vs. normal tissue, corroborate that these genes are involved in the pathogenesis of urinary bladder cancer.

Language

Greek

Subject

RKIP and RAF genes

Real time quantitative PCR

Urinary Bladder Neoplasms

Urinary bladder cancer

Γονίδια RKIP και RAF

Ενδοσκόπηση μέσου ρινικού πόρου

Καρκίνος ουροδόχου κύστεως

Issue date

2012-07-24