Your browser does not support JavaScript!

Home    Search  

Results - Details

Search command : Author="Γανωτάκης"  And Author="Δημήτριος"

Current Record: 46 of 67

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Identifier 000360484
Title Απομόνωση και χαρακτηρισμός του ενζύμου λυάση του υδροϋπεροξειδίου των λιπαρών οξέων, υπερεκφρασμένο σε βακτήρια Escherichia coli
Alternative Title Isolation and characterization of the enzyme hydroperoxide lyase, overexpressed in Escherichia coli bacteria
Author Παναγάκου, Ιωάννα Βασιλείου
Thesis advisor Γανωτάκης, Δημήτριος
Abstract Fatty acid hydroperoxide lyase (HPL), is an important factor of the lipoxygenase path, that is the defense system of plants against exogenous factors. HPL’s function leads to small molecular weight products, of great importance, such as 2-(E)-hexenal and hexanal. In this work, HPL was isolated from E. coli bacteria in which it was cloned, using two different types of nonionic detergents, Triton X-100 and Dodecyl Maltoside (DM). The results of this work showed that, DM is the most appropriate detergent, as it allows further analysis of the protein using spectroscopic techniques. Afterwards, HPL was characterized using circular dichroism and mass spectroscopy, while an attempt isolating the protein directly from olive leaves was carried out. The protein, which was isolated and identified, was a similar in action protein, a peroxidase. Finally, 2-(E)-hexenal and hexanal, two of the HPL’s products, were tested for their antimicrobial, antifungal and anticancer properties. 2-(Δ)-hexenal has an intense effect, while hexanal is less effective.Fatty acid hydroperoxide lyase (HPL), is an important factor of the lipoxygenase path, that is the defense system of plants against exogenous factors. HPL’s function leads to small molecular weight products, of great importance, such as 2-(E)-hexenal and hexanal. In this work, HPL was isolated from E. coli bacteria in which it was cloned, using two different types of nonionic detergents, Triton X-100 and Dodecyl Maltoside (DM). The results of this work showed that, DM is the most appropriate detergent, as it allows further analysis of the protein using spectroscopic techniques. Afterwards, HPL was characterized using circular dichroism and mass spectroscopy, while an attempt isolating the protein directly from olive leaves was carried out. The protein, which was isolated and identified, was a similar in action protein, a peroxidase. Finally, 2-(E)-hexenal and hexanal, two of the HPL’s products, were tested for their antimicrobial, antifungal and anticancer properties. 2-(Δ)-hexenal has an intense effect, while hexanal is less effective.
Language Greek
Subject 2-(E)-hexenal
2-(E)-εξενάλη
Circular dichroism
E.coli
Fatty acid hydroperoxide lyase (HPL)
Hexanal
Mass spectroscopy
Peroxidase
Εξανάλη
Κυκλικός διχρωισμός
Λυάση του υδροϋπεροξειδίου των λιπαρών οξέων
Υπεροξειδίαση
Φασματοσκοπία μάζας
Issue date 2010-09-10
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link https://elocus.lib.uoc.gr//dlib/e/f/9/metadata-dlib-f83832caac40a5402eb21479261023b8_1284629345.tkl Bookmark and Share
Views 69

Digital Documents
No preview available

View document
Views : 9