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Identifier 000358569
Title Απομόνωση και χαρακτηρισμός φωτοσυνθετικών συμπλόκων του οργανισμού και chlamydomonas reinhardtii
Alternative Title Isolation and characterization of photosynthetic complexes from the organism chlamydomonas reinhardtii
Author Κωστομοίρη, Ειρήνη-Δάφνη Ιωάννη
Thesis advisor Γανωτάκης, Δημήτριος
Abstract In the present study, it is performed the isolation of photosynthetic complexes from both spinach and Chlamydomonas reinhardtii cells. Those complexes were fully characterized and immobilized in a calcium alginate matrix. Besides, Chlamydomonas reinhardtii cells were entrapped in the same material (Vilchez, Garbayo, Markvicheva, Galván, & Leon, 2001). The immobilization of the photosynthetic complexes was successful and it was also determined the photosynthetic function maintenance sharply after the immobilization and after one month’s storage at 4°C. One of the most common hydrogels for entrapment is sodium alginate, providing great stability to proteins and membrane systems (Ertesvåg & Valla, 1998). Alginate is quite promising and fulfills a great variety of requirements as safety to the enzyme, easy manufacturing, non-toxicity and low cost (Smidsrod, 1974). This method is ideal for photosynthetic complexes’ immobilization as it preserves them against aging and damages caused from strong illumination.
Language Greek
Subject Chlamydonas reinhardtii
Immobilization of enzymes
Isolation of enzymes
Photosystem II
Ακινητοποίηση ενζύμων
Απομόνωση ενζύμων
Φωτοσυστήμα ΙΙ
Issue date 2010-07-06
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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