E-Locus - Institutional Repository of the University of Crete

Home Search

Results - Details

Search command : Author="Σαμώνης" And Author="Γεώργιος"

Current Record: 49 of 84

[Add to Basket]

Identifier

000374535

Title

Μελέτη της προγνωστικής σημασίας της ανίχνευσης μικρομεταστατικής νόσου στο αίμα και τον μυελό των οστών σε ασθενείς με καρκίνο του παχέος εντέρου σταδίου B2 και C κατά astler coller

Alternative Title

Prognostic significant of the detection of micrometastatic disease in peripheral blood and bone marrow in patient with stage B2 and C colorectal cancer

Author

Βαρδάκης, Νικόλαος Κ.

Thesis advisor

Γεωργούλιας, Βασίλειος

Reviewer

Σαμώνης, Γεώργιος
Χαλκιαδάκης, Γεώργιος
Ζώρας, Οδυσσέας
Μαυρουδής, Δημήτριος
Σουγκλάκος, Ιωάννης
Τζαρδή, Μαρία

Abstract

Purpose: To evaluate the clinical relevance of circulating CEACAM5mRNA-positive cells in patients with operable colorectal cancer (CRC). Methods: Peripheral blood was obtained from 265 patients with operable CRC before the initiation of adjuvant systemic therapy, from 96 normal donors and the Lovo cell line as negative and positive controls, respectively. The detection of CEACAM5mRNA-positive cells was performed using a real time-PCR assay. The association with known prognostic factors and the effect of CEACAM5mRNA-positive cells on patients' prognosis was investigated. Results: The analytical detection limit of the method was found to correspond to 0.7 Lovo cell equivalence/5 μg RNA, with a sensitivity of 1 tumor cell/105 normal cells and a specificity of 97%. Ninety-eight (37%) patients had detectable circulating CEACAM5mRNA-positive cells. Detection of CEACAM5mRNA-positive cells was significantly associated with higher relapse rate (p<0.001), decreased Disease-Free Survival (DFS; p<0.001), higher death rate (p=0.017) and decreased median overall survival (p=0.025). Multivariate analysis revealed that the detection of circulating CEACAM5 mRNA-positive cells was an independent prognostic factor for decreased DFS (HR=3.4; 95% CI: 2.0-5.9; p<0.001). Conclusions: Detection of peripheral blood CEACAM5mRNA-positive cells is an adverse prognostic factor correlated with poor clinical outcome in patients with operable CRC.

Language

Greek

Subject

Colorectal cancer

Gastrointestinal system

Micrometastatic disease

Peripheral blood

Prognostic significance

Stage II III CRC

Καρκίνος παχέου εντέρου

Μικρομεταστατική νόσοι

Ορθοκολικός καρκίνος