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Identifier 000397564
Title Οπτικοί βιοαισθητήρες για την ανίχνευση βιομορίων χωρίς τη χρήση ιχνηθετών
Alternative Title Optical biosensors for label-free detection of biomolecules
Author Ψαρούλη, Αιμιλία Ανδρέα
Thesis advisor Χανιωτάκης, Νικόλαος
Reviewer Βαμβακάκη, Μαρία
Γανωτάκης, Δημήτριος
Θερμού, Κυριακή
Κακαμπάκος, Σωτήριος
Πέτρου, Παναγιώτα
Ράπτης, Ιωάννης
Abstract Biosensors are self-contained integrated devices capable to provide analytical information using biological recognition molecules in direct spatial contact with a transducer. The aim of this thesis was to develop and evaluate an integrated on silicon substrate optical immunosensor based on Mach-Zehnder Interferometry for the immunochemical determination of clinical analytes. The transducer is fabricated entirely by mainstream silicon technology in arrays of ten sensors in a single silicon chip. Each sensor consists of an integrated on silicon light source that emits a broad spectrum in visible-near ultraviolet range coupled to an integrated silicon nitride waveguide which has been patterned into a Mach-Zehnder interferometer. As in the classical Mach-Zehnder interferometers, the waveguide in the proposed sensor is split into two arms, the sensing one which is appropriately modified with recognition biomolecule and the reference arm that is covered by a protective layer. The specific binding of the analyte with the immobilized onto the sensing arm recognition biomolecule causes an effective refractive index change thus changing the phase of the waveguided light with respect to the reference arm. As a result, when the two arms converge again, an interference spectrum is generated that shifts during bioreaction providing the ability to monitor in real-time without using labels. The sensor was evaluated with respect to the determination of analytes of clinical interest and especially for the immunochemical determination of C-reactive protein in human serum samples by immobilizing a CRP specific antibody in the Mach-Zehnders sensing arm. CRP is a marker of inflammation widely used in everyday clinical practice for diagnosis and therapy monitoring of inflammatory situations. CRP has been also proposed as a prognostic marker of myocardial infraction. The immunosensor developed was applied for the CRP determination in serum samples from unknown donors and the values determined were in good agreement with those received for the same samples by commercially available kits and instruments demonstrating the reliability of the determinations performed with the immunosensor developed and its potential for analysis of clinical samples.
Language Greek
Subject C-reactive protein
C-αντιδρώσα πρωτεΐνη
Label-free detection
Mach-Zehnder interferometers
Monolithically integrated on silicon Mach-Zehnder interferometers
Ανάλυςη χωρίς τη χρήση ιχνηθετών
Ολοκληρωμένα σε ψηφίδες πυριτίου συμβολόμετρα Mach-Zehnder
Συμβολόμετρα Mach-Zehnder
Issue date 2015-10-29
Collection   School/Department--School of Sciences and Engineering--Department of Chemistry--Doctoral theses
  Type of Work--Doctoral theses
Permanent Link https://elocus.lib.uoc.gr//dlib/4/9/d/metadata-dlib-1448962588-806297-1087.tkl Bookmark and Share
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