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Identifier 000428741
Title Επίδραση των συνθηκών ανάπτυξης στην παραγωγή υδρογόνου, πολυυδροξυαλκανοϊκών και εξωπολυσακχαριτών σε φωτοσυνθετικούς μικροοργανισμούς
Alternative Title Effects of growth conditions on production of hydrogen, polyhydroxyalkanoates and exopolysaccharides in photosynthetic microorganisms
Author Πολωνιατάκη, Ελένη Γ.
Thesis advisor Γανωτάκης, Δημήτριος
Reviewer Κοτζαμπάσης, Κυριάκος
Παυλίδης, Ιωάννης
Abstract In the present work, the effect of growth conditions on the productions of three natural products, hydrogen, polyhydroxyalkanoate and exopolysaccharides, by photosynthetic microorganisms were investigated. This thesis is divided into two sections. In the first section, the effects of three organic acids, acetate, succinate, and malate, on the production of hydrogen and poly-β-hydroxybutyrates was thoroughly studied. Growth of the microorganism Rhodopseudomonas palustris sp. under two growth conditions, demonstrated that the presence of sulfur favors the production of hydrogen, while its absence boosts the production of poly-β-hydroxybutyrate. In the second part of the thesis, the effect of a carbon source on the production of exopolysaccharides by the cyanobacterium Nostoc calcicola was investigated. The examined carbon sources were either an organic source, glucose, or two inorganic sources, sodium carbonate and carbon dioxide. Experiments were performed under stress conditions for the cells, which was achieved by removing nitrogen from the growth medium. Furthermore, EPS production was studied in four different growth conditions. Specifically, in the presence of all components, in the absence of nitrogen, in the absence of both nitrogen and carbon, and in the absence of sulfur. All experiments were conducted in the presence of a continuous flow of air. Finally, the cells were studied by optical and electron microscopy in the aforementioned conditions.
Language Greek
Subject Cyanobacteria
Exopolysaccharides (EPS)
Nostoc calcicola
Polyhydroxyalkanoates (PHB)
Rhodopseudomonas palustris sp.
Rhodopseudomonas palustris sp. υδρογόνο
Εξωπολυσακχαρίτες (EPS)
Πολυυδροξυαλκονοϊκά (PHB)
Φωτοσυνθετικοί μικροοργανισμοί
Issue date 2020-03-27
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Chemistry--Post-graduate theses
  Type of Work--Post-graduate theses
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