Your browser does not support JavaScript!

Home    Search  

Results - Details

Search command : Author="Κεντούρη"  And Author="Μαρουδιώ"

Current Record: 9 of 32

Back to Results Previous page
Next page
Add to Basket
[Add to Basket]
Identifier 000403552
Title Καλλιεργούμενα βακτηριακά στελέχη από το υποθαλάσσιο ηφαίστειο Κολούμπο: ανθεκτικότητα σε αντιβιοτικά, βαρέα μέταλλα και υψηλή οξύτητα
Alternative Title Cultivated bacterial strains from the submarine volcano Kolumbo: resistance to antibiotics, heavy metals and high acidity
Author Γαβριηλίδου, Ασημένια Θ.
Thesis advisor Κεντούρη, Μαρουδιώ
Reviewer Κωτούλας, Γεώργιος
Καθάριος, Παντελής
Abstract The extreme environments of Greece are dynamic and highly diverse showing great ecological interest. However, they still remain unexplored and inadequately studied. The extreme environmental conditions prevent the living of higher organisms. Nevertheless, microorganisms are capable of adjusting in hostile conditions and they are expected to develop unique characteristics conferring resistance. Moreover, this adaptation is suspected to induce the multiresistance of microorganisms against different types of chemical/physical stress. This hypothesis is examined in this study. We investigated the resistance of cultivated bacteria isolated from the water column above the hydrothermal vent field of the submarine volcano Kolumbo of Santorini. This region is unique because of the discharge of CO2-rich hydrothermal fluids, the high acidity within the crater and the rich metal deposits. 54 bacterial strains belonging to three different genera were examined for their resistance to 4 heavy metals, 6 antibiotics, a pH range (from 3.6 to 9.7) and olive oil mill waste with high content of toxic polyphenolic compounds. The applied method is based on monitoring the bacterial growth by measuring the optical density of bacterial subcultures containing serial dilutions of the studied antimicrobial agent in 384-well microplates. We compared the antimicrobial resistance of the bacteria isolated from different depths of the water column, according to the minimum concentration that inhibits 50% of the bacterial growth (MIC50). The bacterial strains from the deeper layer of the water column showed higher resistance to arsenic, antibiotics and lower pH values compared with the bacteria from shallower waters. Furthermore, a higher percentage of the strains from the deeper waters managed to grow in 50%, 25% and 12.5% v/v of olive oil mill waste in relation to the shallower strains. To our knowledge, this is the first report of correlation between antibiotic and high acidity resistance. The results support the fact that Kolumbo’s unique environment has a great impact on its microbial inhabitants. Therefore, Kolumbo serves as an excellent “underwater natural laboratory” for the investigation of important ecological questions and different phenomena, such as the ocean acidification.
Language Greek
Subject Antimicrobial resistance
Extreme environments
Ακραία περιβάλλοντα
Ανθεκτικότητα μικροοργανισμών
Issue date 2016-11-18
Collection   Faculty/Department--Faculty of Sciences and Engineering--Department of Biology--Post-graduate theses
  Type of Work--Post-graduate theses
Permanent Link Bookmark and Share
Views 42

Digital Documents
No preview available

View document
Views : 2